Jurnal Ilmu dan Teknologi Kelautan Tropis
Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis

THE HISTOLOGICAL, EXTRACTION AND CHARACTERIZATION COLLAGENS YELLOW-PIKE CONGER Muarenesox talabon

Dewi Setiyowati Gadi (Departemen Teknologi Hasil Perairan, FPIK-IPB, Bogor)
Wini Trilaksani (Departemen Teknologi Hasil Perairan, FPIK-IPB, Bogor)
Tati Nurhayati (Departemen Teknologi Hasil Perairan, FPIK-IPB, Bogor)



Article Info

Publish Date
02 Jan 2018

Abstract

By product Muarenesox talabon swim bladders can be used as a raw material for desperately needed in the food, biomedical, pharmaceutical, and cosmeceuticals industries. The aims of the research were to observed the histological and determine chemical characteristics of swim bladder including proximate and amino acids; extraction of acid soluble collagen and determine the characteristics of collagen including proximate, pH, heavy metals, microbial, amino acids, functional groups, molecular weights, and thermal stability. The morphology of cunang swim bladder consists of outer, middle, and inner layers containing collagen fibers; 33.67±0.71%wb and protein whichwere dominated by three amino acids that were glycine, proline, and alanine. Pretreatment by 0.1 M NaOH for 8 hours (K1T4) and acid extraction by 0.25 M acetic acid for 72 hours (M1T3) was the best treatment yielding 14.51± 0.43% of collagen; having 12.12±0.04% wb of moisture; 88.54 ± 0.08% wb of protein; 1.31±0.23% wb of fat; 0.17±0.03% wb of ash. Not detected any heavy metals (Pb, Hg, As, Cd). Acidity pH was 4.31 and negative of E. coli and Salmonella. The main amino acids detected were glycine 241.06 mg/g; proline 88.73mg/g; and alanine 86,98 mg/g; FTIR spectra were revealed the presence of triple helix structures; electrophoresis patterns consisted of 136 kDa of mol weight of α1 and 117 kDa of mol weight of α2 were characterisedto be type I collagen; which had Tmax of 195.59ºC and ΔH 7.8113 J/g. Keywords: acid extraction, swim bladder, collagens, thermal stability

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Journal Info

Abbrev

jurnalikt

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Library & Information Science

Description

Aims and Scope Journal of Tropical Marine Science and Technology (Jurnal Ilmu dan Teknologi Kelautan Tropis) is a scientific journal in the field of tropical marine science and technology. We have a aims and scope to focus in publishing a good quality scientific articles for dissemination of ...