NICHE Journal of Tropical Biology
Vol. 3, No. 1, Year 2020

THE TOOL OF RESISTANCE MONITORING OF PLUTELLA XYLOSTELLA, (L.) AND ITS FIELD VALIDATION USING EMAMECTIN BENZOATE AND DIAGNOSTIC CONCENTRATION DETERMINATION

Tarwotjo, Udi (Unknown)
Situmorang, Jesmandt (Unknown)
Martono, Edhy (Unknown)
Rahadian, Rully (Unknown)



Article Info

Publish Date
01 Jun 2020

Abstract

In Indonesia, cabbage pest control is still heavily dependent on the use of insecticides. Although insecticides have many advantages, i.e., reducing the populations of pest quickly, easy to use, and economically advantageous, but it has the negative impact especially the emergence of resistant pest species.The objective of this study are: (1) to monitore the resistance of Plutella xylostella population toward insecticide residues of emamectin benzoate by diagnostic concentration determination, (2) to validate the diagnostic concentration in the field. P. xylostella population were collected from some cabbage farms in Central Java i.e., Bandungan, Sumowono, Cepogo, Kejajar, Kertek, Tawangmangu, Plalar, Puasan, Kaponan, and Keteb.Determination of LC90 was tested using probit analysis. The results showed that five of the eleven tested populations have sensitivity c2 value < c2 table, namely population Selo, Gedongsongo, Gondosuli, Kejajar, and Plalar. The determinated diagnostic concentration is LC90 = 2443.99 ppb with the lowest concentration limits 1213.67 ppb and the highest is 4921.53 ppb, because of the testing results of the sensitivity of all tested populations are still susceptible to emamectin benzoate with mortality > 90% at a concentration of 2000 ppb. The results of validation diagnostic concentration (2443.99 ppb), indicating the mortality percentage caused by eleventh population ranged from 82.76% to 95%, the value of c2 value is smaller than c2 table (df = 1; ? = 0.05) = 3.84. Therefore the diagnostic concentration (ppb 2443.99) is valid for effective monitoring tool for the development of resistance of P. xylostella population. The conventional method to monitor resistance is less sensitive to the resistance emerging occurrence. Therefore, it is necessary to develop monitoring methods which are more sensitive to small changes in the frequency of resistance, by using diagnostic concentration.

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Journal Info

Abbrev

niche

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

NICHE Journal of Tropical Biology is an open access journal that publishes original research across all areas of life sciences in tropical region. The objective of the journal is to disseminate all information across fields of biology as well as to contribute to the understanding and development of ...