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All Journal Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Biosaintifika: Journal of Biology & Biology Education NICHE Journal of Tropical Biology Proceedings of the International Conference on Applied Science and Health
Rully Rahadian
Laboratorium Ekologi dan Biosistematika Jurusan Biologi FSM Undip
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Mathematics Medicine & Pharmacology Nursing Public Health

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Application of Snakehead Fish to Increase Liver Function of Rats after Experiencing Physiological Stress Sunarno, Sunarno; Mardiati, Siti Muflichatun; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13957

Abstract

Physiological stress due to nutritional deficiency accompanied by excessive activity can cause problems of energy deficiency required by the body. Energy shortage can affect the body weight, as well as the weight and histology of the liver. This study was conducted to examine and analyze the effect of snakehead fish meat supplementation in feed toward body weight, weight and hepatic histology in animals experiencing physiological stress. This study used 20 male Wistar rats consisting of 5 treatments with 4 replications. The treatment in this study consisted of control (P0) and treatment (P1: 5%, P2: 10%, P3: 15%, P4: 20%) groups. This research used Completely Randomized Design (RAL). The variables measured in this study were body weight, hepatic weight, central venous diameter and hepatocytes in the test animals. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance. The result of data analysis showed that fish snakehead fish supplementation in feed gave a significant effect to body weight, weight and histology of animal liver. Concentrations of snakehead fish supplements can increase the body weight, hepatic weight, and hepatic histology which was indicated by higher central venous and hepatocyte diameter values compared to other controls and treatments. The conclusion of this research is the provision of snakehead fish supplement (Channia striata) can increase the body weight, hepatic weight, central venous and hepatocytes diameter of Wistar rats with the most influential concentration of 20%. This evidence is useful in studying the mechanism of liver repair due to stress
The Role of Acetylcholine Esterase in Resistance Mechanism of Plutella xylostella to Emamektin Benzoate Tarwotjo, Udi; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13955

Abstract

One of the resistance mechanism of P. xylostellato emamektin benzoate is target insensitivity which is acetylcholine esterase that responsible for resistance occurrence. The objective of this study was to determine the role of acetylcholinesterase in the resistance mechanism of P. xylostella population to emamektin benzoate. For enzyme activity analysis, larvae homogenate of the third instar of P. xylostella was prepared. The number of insects required for each scour is 1 for each field population. The protein content in P. xylostella homogenate was measured by the Folin-Ciocalteu test. Non-specific esterase activity with an absorption rate was read using ELISA reader tool with λ = 450 nm. The inhibition level of acetylcholinesterase activity by emamectin benzoate in the tested population was 36.84%. The highest inhibition occurs in Puasan (Ngablak) population.  The result shows that a α-naphthyl acetate substrate was used so that it was recorded as non-specific esterase activity and did not exhibit esterase activity which specifically describes emamectin benzoate. Non-specific esterase enzyme activity of either α or β-naphthyl acetate substances to benzoic emamectin in the tested population most of the population was still susceptible. On α-naphthyl acetate substrate, the highest absorbance value found in susceptible population to benzoate emamectin (0.773), while the lowest found in Babrik (Ngablak) population  (0.083).
THE POPULATION OF FLIES IN CIKOLOTOK LANDFILL, PASAWAHAN DISTRICT, PURWAKARTA REGENCY, INDONESIA Sugiarto, Danang Wahansa; Hestiningsih, Retno; Rahadian, Rully
Proceedings of the International Conference on Applied Science and Health No 1 (2017)
Publisher : Proceedings of the International Conference on Applied Science and Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.539 KB)

Abstract

Background: The flies act as mechanical vectors of disease to the digestive tract, such as cholera, typhoid, dysentery, and others. There are a lot of flies in a variety of habitats, one of the preferred habitat is on landfills. The first step to controlling flies is a preliminary survey with collecting data about the population of flies. In the Cikolotok landfill, it has never done a survey population of flies. Aims: This study aims to look the number of flies and the priority area for fly control, the most active time of flies, and species of flies in Cikolotok landfill, Pasawahan District, Purwakarta Regency, Indonesia. Methods: This study was an observational-descriptive research. The flies were observed based on the fly that caught by the flypaper trap and cone trap mounted on 3 areas, i.e. area of sorting garbage, area of stockpiling garbage and area of waste treatment. Data were analyzed by univariate method. Results: The results showed that the density of flies in Cikolotok landfill per point range between 0- 179 flies/15 minutes in every 2-hour. The area that had the highest number of flies is area of sorting garbage with 528 flies. The most active time of flies was in a span of 7:00-9:00 am. The species of flies were found are Chrysomya megacephala (52%), Musca domestica (45%) and Sarcophaga bullata (3%). Conclusion: Sorting garbage area has the highest number of flies and C. megacephala more found in landfill because of physical condition of garbage and the condition of area itself. The flies are more active in a span of 07:00-09:00 due to the condition of physical environment. Monitoring, fly control, research about fly-borne disease and relocation of cattle are needed to control the flies and the effects. 
Utilization of Snakehead Fish Meat for Increasing Kidney Function in Rat Experiencing Physiological Stress Sunarno, Sunarno; Sokmawati, Hajar; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.213 KB) | DOI: 10.15294/biosaintifika.v10i3.16254

Abstract

Physiological stress for a long time can lead to impaired kidney organ function. Snakehead fish meat are known to contain important nutrients such as albumin, glutamine, glycine, cysteine, and various minerals that can improve kidney function. This study was conducted to examine and analyze the effect of snakehead fish supplementation in feed towards body weight, kidney weight, diameter and number of glomerulus in Wistar rats with physiological stress. This study used 20 male Wistar rats divided into 5 groups consisted of 1 control group (P0) and 4 treatment groups (P1: 5%, P2: 10%, P3: 15%, P4: 20%). The treatments were performed with 4 times repetition. This study used Completely Randomized Design (CRD). The variables measured were body weight, kidney weight, diameter and number of renal glomerulus. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance level. The results of this study were: the supplementation of snakehead fish with 20% of concentration can increase the body weight; concentration 10; 15; and 20% can increase the weight of the kidney; concentrations of 5%, 10%, 15%, and 20% can increase the glomerular diameter in Wistar rats with physiological stress. The supplementation of snakehead fish with concentration of 5% to 20% did not affect the number of glomerulus of Wistar rats with physiological stress. Results of this study is useful as information for people who study the mechanism of kidney repair due to physiological stress.
The Effectiveness of Plastic Mulch for Maintaining the Potato Farmland in Dieng Plateau Using Soil Biological Quality Index Agustina, Dian; Tarwotjo, Udi; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.17804

Abstract

Dieng farmers apply plastic mulch to reduce erosion and to prevent the loss of nutrients. This study aimed to determine the effectiveness of plastic mulch in maintaining the quality of potato farmland using the Soil Biological Quality Index (QBS-ar) based on the existence of microarthropods. Soil sampling was conducted in six different locations, i.e., flat area with mulch, sloped area with mulch, the flat area without mulch, sloped area without mulch, flat forest area, and sloped forest area. Microarthropods were obtained from soil samples using a Berlese Funnel method. Soil quality was analyzed using QBS-ar index which was calculated based on Eco-morphological Index (EMI). The results showed that the value of QBS-ar index in potato farmland with the use of plastic mulch was much higher than in farmland without plastic mulch in bothflat and sloped area. Compared to the soil in the forest area, the value of QBS-ar index in farmland with the use of plastic mulch, especially in the flat area, was relatively similar. Hence, the use of plastic mulch for potato cultivation in the hilly area is effective in maintaining soil quality. This finding would be beneficial for developing a soil conservation strategy to achieve sustainable agriculture in Dieng agricultural area.
Cinnamon and Gotu Kola Supplementation to Produce High Antioxidant and Low Cholesterol of Quail Pectoral Meat Sunarno, Sunarno; Zubaedah, Siti; Rini, Almalina Nabila Sulistyo; Sekarsari, Eryanti; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18220

Abstract

Cholesterol in quail meat is one of the factors causing consumers to control consumption of quail meat. On the other hand, the antioxidant content in quail meat could attracts the consumers. Cinnamon (Cinnamomum sp.) bark and gotu kola (Centella asiatica) leaves are medicinal plants that contain many polyphenol compounds. The objective of this study was to obtain the most optimum formula made from cinnamon and gotu kola as a feed supplement to produce the quail meat which has low cholesterol but rich in antioxidants. This study used a completely randomized design which consisting of six groups with three replications. The six groups included one control (P0), 5 treatments consisted of 5% cinnamon (P1), 5% gotu kola (P2), combination of cinnamon and gotu kola powder with ratio 5%:5% (P3), 5%:10% (P4) and 10%:5% (P5).  The results showed that food supplements made from cinnamon bark and gotu kola leaves could increase the body weight, antioxidant levels, and reduce cholesterol levels in quail meat. Cinnamon as feed supplement with a concentration of 5% or a combination of cinnamon and gotu kola with a ratio of 5%: 10% provided the best effect on antioxidants and cholesterol level in meat. The use of cinnamon and gotu kola supplementation to produce high antioxidant and low cholesterol of quail met have not studied yet. The result of the study would be beneficial for developing the healthy, safe and good quality of quail meat.
The Dominance of Tramps Ants in The Settlement Area of Semarang, Central Java Putra, Ivan Mahadika; Hadi, Mochammad; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.21026

Abstract

The high rate of urbanization in Semarang have caused the land conversion from forest into various human need spaces. The land conversion is not only affect the life of humans but also the animals, including ants. The objectives of this study were to examine the dominance of tramp ants in the settlement area of Semarang City. The method used was the bait trap method using chicken intestine as a bait. Samples of ants were collected from four habitats, i.e., waste disposal area, traditional markets, house area, and urban parks. This study found four dominant ants, i.e., Anoplolepis gracilipes, Paratrechina longicornis, Solenopsis geminata and Monomorium pharaonis. Two of them were categorized as tramp ants (Anoplolepis gracillipes and  Paratrechina longicornis). The highest diversity of ants was found in house  (H'= 2.72).  The waste area tends to be an ideal habitat for particular species, which was proved by the low value of Simpson's dominance index (iD = 0.06) and a high value of evenness index (E=0 0.89). This study provides new information about tramp ants population as pest in urban area. The findings would be beneficial for controlling the population of tramp ants, which are disturbing humans in the settlement area.
Struktur Komunitas Mikroartropoda Bryofauna Terestrial Di Zona Tropik Gunung Ungaran, Semarang, Jawa Tengah Munarsih, Atik; Rahadian, Rully; Hadi, Mochamad
Bioma : Berkala Ilmiah Biologi Vol. 16, No.1, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.492 KB) | DOI: 10.14710/bioma.16.1.50-57

Abstract

Bryofauna is all of the animal life which associated with moss. Actually, biodiversity of bryofauna have not been studied much in Indonesia. Research on community structure of terrestrial microarthropod bryofauna has been done in three different altitudes in the tropical zone of the Ungaran Mountain, Semarang, Central Java. The objective of this study is to compare community structure of bryofauna contained in three different altitudes in the tropical zone. The research was conducted from April to November 2012. Sampling was carried out at 3 stations with the different heights that are the station I with an altitude 750 m asl, the altitude of the station II with an altitude 980 m asl and the station III with an altitude of 1100 m asl. Sampling was done purposively using square plot method. Bryofauna identification was conducted in Ecology and Biosistematics Laboratory University of Diponegoro and Entomologi Laboratory of Zoology Departement Indonesian Institute of Sciences. The results shows that the tropical zone have5 classes, 16 orders and 31 suborders/family of bryofauna. Mesostigmata and Oribatida was the dominant taxa at all heights except at an altitude of 980 m asl, Oribatida was categorized as subdominant. Diversity of bryofauna at different heights shows that decreasing diversity patterns, along with the increasing altitude. In general, the distribution of bryofauna at different heights is quite spread evenly with the flattening index values ​​betweens 0.84 to 0.94. Bryofauna taxsa richness and diversity of bryophytes at different heights in the tropical zone showed the same pattern fluctuated. Taxa group which play a role as predator are taxa that the most abundant in the tropical zone of the Ungaran Mount.   Keywords:Bryofauna terrestrial, bryophytes, Ungaran mount, community structure
Struktur Komunitas Mikroartropoda Tanah di Lahan Pertanian Organik dan Anorganik di Desa Batur Kecamatan Getasan Salatiga Husna, Shifa Aulia; Hadi, Mochamad; Rahadian, Rully
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.369 KB) | DOI: 10.14710/bioma.18.2.157-166

Abstract

Soil microarthropods is a arthropods that have an important role in decomposing organic materials and soil nutrients. On the farmland there is organic materials content and soil nutrients that abundants enough, because the addition of manure as a source of energy in the ground. The research was conducted in August-October 2015 took place in the field of organic and inorganic farmland in the Batur Village, Getasan Sub-district, Salatiga. This study aimed to examine the soil microarthropods community structure in organic and inorganic farmland as well as the effect of chemical and physical environmental factors to community structure of soil microarthropods. The study was conducted with samples of soil sampling method (PCT) and extracted using Barlese Funnel Extractor. Statistically show that diversity of soil microarthropods between organic and inorganic farmland are not significantly different. There is found 28 taxa of soil microarthropods in organic farmland and 23 taxa in inorganic. The highest total individual density of the taxa are exist in the organic farmland (2260 individual/ m²). The highest abundance of soil microarthropods taxa in inorganic farmland are Carabidae (26,55%) dan Prostigmata (13,27%), while in inorganic farmland are Carabidae (17,24%) dan larva Coleoptera (13,79%). The evenness of soil microarthropods taxa in organic farmland are much low compared with inorganicfarmland, because there is a dominant taxa (Carabidae). There is an influence relation between the chemical and physical environmental factors including water content, porosity, nutrient, and organic materials with community structure of soil microarthropods. Key words:Community structure, Soil microarthropods, Organic and inorganic farmland
Struktur Komunitas Mikroartropoda Tanah di Lahan Penambangan Galian C Rowosari, Kecamatan Tembalang, Semarang Larasati, Wiatri; Rahadian, Rully; Hadi, Mochamad
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.509 KB) | DOI: 10.14710/bioma.18.2.79-88

Abstract

Rowosari miningsite isthe ‘C’ type of excavation area in Semarang. Mining activities may affect environmental disturbance, including on soil fauna. This research aims to determine the community structure of soil microarthropods in Rowosari mining area. The research was conducted in August-September 2015. Soil samples were taken on diagonal plot of 5x5 m2 with a five-point sampling on two stations, namely Post Mining Area (PoMA) and Pre Mining Area (PeMA). The analysis used in the research are relative abundance index, Shannon-Weiner diversity index, evenness index, and Sorensen similarity index. The results shows that there are 360-660 individuals/m2 from 10 ordo and 24 taxa found in Rowosari Excavation Site. The highest relative abundance index is Carabidae (22,22) in PoMA station and Prostigmata (21,21) in PeMA station. The diversity both station belongs to medium category (2,43-2,45). The highest evenness index is 0,95. The similarity taxa of soil microarthropods in two stations are categorized as medium. Community structure of soil microathropods in Post Mining Area and Pre Mining Areawere no significant differences. Keywords: Community structure, Soil microarthropods, Miningexcavation C.
Co-Authors Amelinda, Elda Dheiva Atik Munarsih, Atik Dian Agustina Dian Ratna Sari Edhy Martono, Edhy Ervianna, Ana Rita Husna, Shifa Aulia Jesmandt Situmorang Karyadi Baskoro Latifah Fitria Andriani Lilih Khotim Perwati Mochamad Hadi Mochammad Hadi Murtiati, Sri Niswah, Rofiatun Nugroho, Nindya Putra Wahyu Putra, Ivan Mahadika Retno Hestiningsih Rini, Almalina Nabila Sulistyo Roma Witriyanto, Roma Sekarsari, Eryanti Siti Muflichatun Mardiati siti zubaedah Sokmawati, Hajar Sugiarto, Danang Wahansa Sunarno Sunarno Tamar, Irsyad Muhammad Udi Tarwotjo, Udi Wiatri Larasati, Wiatri