Journal of the Indonesian Tropical Animal Agriculture
Vol 38, No 2 (2013): (June)

IDENTIFICATION OF MELANOCORTIN 1 RECEPTOR (MC1R) GENE BASED ON COAT COLOR OF BALI COWS OF KUPANG BY USINGTHE PCR-RFLP METHOD

Tabun, A.C. (Unknown)
Hartatik, T. (Unknown)
Sumadi, S. (Unknown)

Article Info

Publish Date
15 Jun 2013

Abstract

The objective of this study was to identify the Melanocortin 1 Receptor (MC1R) gene in sorrel-,black- and white-Bali cows originated from Kupang-East Nusa Tenggara. Blood samples were takenfrom 46 cows consisted of of 17, 18 and 8 of sorrel, black and 8 heads, respectively, and 3 heads of bull.The PCR-RFLP method was performed to identify the gene. Amplification of PCR gene MC1R ofKupang's Bali Cattle (KBC) was 296 bp. The digestion by using MspI enzyme showed that there wereEE genotype (169, 136 bp) in Bali bull, sorrel and white cows. Two 2 genotypes, those were EE (169,136 bp) and Ee (296, 169, 136 bp) were found in black Bali cow. The frequency of E alele of KBC was0.99 (99%) and e alele was 0.01 (1%). MC1R gene in KBC was monomorphic and the it can be used asthe marker of the coat color in the population.

Copyrights © 2013




Citation Download
RIS
EndNote, Reference Manager, ProCite
BibTex
Latex, Jabref
Original Source
Download Original
Google Scholar
Check in Google Scholar
Journal Info
Journal of the Indonesian Tropical Animal Agriculture
Website

Abbrev

jitaa

Publisher

Universitas Diponegoro

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research ...