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Edy Kurnianto
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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Articles 591 Documents
INHIBITION OF Escherichia coli O157:H7 CONTAMINATION ON CHICKEN MEAT BY NATURAL VINEGAR PREPARED FROM BANANA PEEL AND COCONUT WATER Miskiyah, M.; Juniawati, J.; Andriani, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 1 (2016): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.1.21-27

Abstract

The use of vinegar as a natural preservative to inactivate microbial growth in meat was investigated. Vinegar was prepared from banana peel and coconut water, and the efficacy against Escherichia coli O157:H7 was tested. Chicken meat were treated with vinegar solution (equals to 1% acetic acid), acetic acid solutions (1%), and lactic acid solutions (2%), control (distilled water). Samples were soaked with acid solution treatment for 1 minute (1.125:1 w/v; chicken meat: solution treatment). Treated samples were inoculated with E. coli O157:H7 on the surface of the chicken meat, then left for 20 minutes for absorption of bacteria into the meat. Observation was done at 0, 3, 6, 9, and 12 days at low temperature storage, and at 0, 6, 12, 18, 24 hours at room temperature storage. The results showed that banana peel vinegar and coconut water vinegar were effective as natural preservative. In conclusion, the vinegar were potentially inhibited E. coli O157:H7 growth at chicken meat until 12 hours at room temperature storage and 9 days at cold temperatures.
EFFECTS OF Citrus hystryx AS FAT PROTECTOR ON UNSATURATED FATTY ACIDS, CHOLESTEROL AND CHEMICAL COMPOSITION OF LAMB MEAT Tiven, N.C.; Siwa, I. P.; Joris, L.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 1 (2016): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.1.45-49

Abstract

This study was conducted to determine the effect of Citrus hystrix as fat protector on unsaturated fatty acids, cholesterol and chemical composition of lamb meat. The research design applied was completely randomized design with 3 treatments and 5 replications. Fifteen local male lambs aged 9-12 months weighing 14-17 kg, were divided into 3 groups of different diet treatments (P0 : sheeps were only given a basal diet; P1 : sheeps are given basal diet and 3% cooking oil; P2 : sheeps are given basal diet, 3% cooking oil and protected by 3% Citrus hystrix powder). The data were analyzed by analysis of variance, the differences among treatments were tested by Duncan’s New Multiple Range Test. The results showed meat from sheeps that consume P2 diet has a total fatty acid and polyunsaturated fatty acids content were higher (P
COLOR, PIGMENT AND RESIDUAL NITRITE OF DENDENG SAPI NATURALLY CURED AT VARIOUS LEVEL OF CELERY LEAVES AND INCUBATION TEMPERATURES Saputro, E.; Bintoro, V. P. Priyo; Pramono, Y. B. Budi
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 2 (2016): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.2.99-105

Abstract

his study was conducted to analyze the effect of natural curing by various levels of fresh celery leaves (FCL) and various levels of incubation temperature. The incubation temperature was room temperature (RT) and temperature of 40.6oC (40.6) for 2 hours. These incubation temperatures for creating dendeng sapi were designed to provide the similar curing characteristics of the control (by addition of 50 ppm of NaNO2). Four naturally cured dendeng sapi treatments were A1 = 22 g FCL/kg of beef, RT; A2 = 36 g FCL/kg of beef, RT; A3 = 22 g FCL/kg of beef, 40.6; A4 = 36 g FCL/kg of beef, 40.6 and a treatment of conventionally cured dendeng sapi by addition of 50 ppm NaNO2 as a control (A0 ). All of the naturally cured dendeng sapi (A1-A4) showed color, cured pigment, total pigment and residual nitrite which were not significantly different compared with A0 . The FCL used in this study was an effective replacement for sodium nitrite at the test levels in manufacturing natural cured dendeng sapi. These results indicated that at fewer FCL levels (22 g/kg of beef) and a more practical of the incubation for 2 hours at room temperature were more effective to create naturally cured dendeng sapi.
ESTIMATION OF MOST PROBABLE PRODUCING ABILITY VALUE FOR CALF BIRTH'S PERFORMANCE IN SUMBA ONGOLE COWS Said, S.; Agung, P. P.; Putra, W. P. B.; Anwar, S.; Wulandari, A. S.; Sudiro, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 2 (2016): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.2.53-60

Abstract

An estimation of most probable producing ability (MPPA) value based on the calves performance is very important for the cow selection. The birth data record of 48 Sumba Ongole (SO) cows and record data from 52 callf birth’s performance were used to estimate the value of MPPA. High of r value (r > 0.30) was obtained at the body length (BL). Birth weight (BW), withers high (WH) and chest girth (CG) had a moderate value (0.10 < r < 0.30) of repeatability. The MPPA value of the cumulative calf birth’s performance of cows based on the highest birth records was 4.64 (cow number 3770) and the lowest was -4.64 (cow number 2283). Calf number B2076 (cow number 3586) had the highest ratio of WH (115.70); BL (124.26); CG (118.90), while the highest ratio of BW (168.35) was obtained in calf number B2095 (cow number 3731). It was concluded that the BW of birth had a moderate value of r (0.10) and could be used as a selection criteria accurately. 
ASSOCIATIONS BETWEEN POLYMORPHISM OF GROWTH HORMONE GENE WITH MILK PRODUCTION, FAT AND PROTEIN CONTENT IN FRIESIAN HOLSTEIN CATTLE Hartatik, T.; Kurniawati, D.; Adiarto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.133-137

Abstract

The aim of the research was to determine the associations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production of Friesian Holstein Cattle. A total of 62 cows which consist of two Friesian Holstein cattle groups (from New Zealand=19 heads and Australia=43 heads) were used for the research. We performed the Polymerase Chain Reaction and followed by Restriction Fragment Length Polymorphism using AluI restriction enzyme. The genotype was observed base on the target gene GH 211 bp in the part of intron 4 and exon 5 of growth hormone gene. The frequencies of genotypes LL were found higher than genotype LV in both groups. Friesian Holstein cattle from New Zealand showed the genotype LL and LV as 84% and 16%, respectively. Friesian Holstein cattle from Australia show the genotype LL and LV as 79% and 21%, respectively. The association between Leu/Val polymorphism on milk production, fat and protein content in both groups did not show the significant effect. Base on two groups of the origin of cattle, the result showed the significant different on fat and protein content of milk. Fat and protein contents of milk were higher in breed of FH imported from Australia compared to those in breed of FH imported from New Zailand.
HERITABILITIES OF BODY SIZE BY GROWTH HORMONE (GH-Msp1) GENOTYPES USING PCR-RFLP IN ONGOLE GRADE CATTLE Paputungan, U.; Hakim, L.; Ciptadi, G.; Lapian, H. F. N.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.138-144

Abstract

Genotypic performance in term of heritability as the crucial factor of animal economical traits for body size inheritance had not been fully studied in Ongole-grade cattle. The objectives of this research were to define the heritability values of live weight, chest girth and body length in Ongole-crossbred cattle. Total of 37 blood samples were collected from parental cows and 2 blood samples from parental Ongole breed bulls. All blood samples were screened for the presence of growth hormone (GH) locus using PCR-RFLP method involving restricted enzyme Msp1 on agarose-gel (1.2%). Data were analyzed using statistical program in Excel XP. Results showed that the phenotypic estimation average of Ongole grade cattle population of live weight, chest girth and body length were 445.41 ± 45.95 kg, 175.35 ± 4.11 cm, and 139.70 ± 5.73cm, respectively. The heritability values of animal live weight, chest girth and body length in this study were 0.24, 0.003, and 0.41, respectively. These heritability values of animal live weight and body length would be categorized as moderate to high genotypic performance values, while the heritability of animal chest girth was included in low heritability standard of the animal economical trait performance.
STUDY ON CHANGES OF SPERM HEAD MORPHOMETRY AND DNA INTEGRITY OF FREEZE-DRIED BOVINE SPERMATOZOA Said, S.; Afiati, F.; Maulana, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.145-152

Abstract

Changes of sperm heads morphometric and DNA integrity of freeze-dried bovine spermatozoa were investigated. Freeze-dried spermatozoa had stored in the refrigerator at 4°C for 2 years. Computer-assisted sperm analysis (CASA) was used in this study to identify sperm head morphometry, while for DNA integrity analysis using acridine orange staining. Samples were smeared on glass slides, fixed for 2 h in acetic alcohol and stained with acridine orange solution. After staining, each slide was examined at x400 magnification in a fluorescence microscope with axio vision (Zeiss Company, Germany). Proportion of fluorescence red and green emissions of the sperm head were examined and scored. These results indicated that sperm head had enlarged significantly (P<0.05) after freeze-drying process. However, freeze-dried sperm heads morphometry significantly (P<0.05) decrease after incubation for 3 and 6 hours. Changes of DNA integrity of freeze-dried spermatozoa significantly (P<0.05) decrease after incubation for 6 hours. In the present study concluded that (1) freeze-drying spermatozoa caused sperm head morphometric enlarged, whereas incubation time caused sperm heads decreased, (2) DNA integrity of freeze-dried sperm head is still intact during incubation 3 hours, and decreased DNA integrity occur in incubation for 6 hours.
COMBINATION OF SOYBEAN MEAL AND Hibiscus tiliaceus LEAF IN THE GOAT DIET: EFFECT ON SOME PARAMETERS OF CARBOHYDRATE METABOLISM Armayanti, A. K.; Nuswantara, L. K.; Achmadi, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.153-158

Abstract

The purpose of this study was to clarify effect of the combination of waru leaf (Hibiscus tiliaceus) sapponin with soybean meal protein in the goat diet on some parameters of carbohydrate metabolism. The study used 16 Ettawah cross bred male goats with body weight average of 16 kg and aged at 7 months. The dietary treatments were: T0 = total mixed ration (0.09% of Hibiscus tiliaceus leaf sapponin, 8.69 % of CP, 62.11% of TDN), T1 = T0 + 3% soybean meal protein, T2 = T0 + 6% soybean meal protein, and T3 = T0 + 9% soybean meal protein. The treatments were allotted in a completely randomized design, with 4 treatments and 4 replicates of each. The results showed that dietary treatments did not effect significantly (P>0.05) on intakes of dry matter, carbohydrate, energy, ruminal butirat and valerat concentrations. Crude protein intake, feed digestibility, ruminal acetate and propionate concentrations, ratio of non-glucogenic to glucogenic VFAs, and estimated methane production were increased (P<0.05) by dietary experiments. The protein supplementation in a Hibiscus tiliaceus leaf contained diet should be balanced with the energy level.
THE EFFECT OF ENERGY LEVEL OF FEEDING ON DAILY GAIN, BLOOD GLUCOSE AND UREA ON MADURA CATTLE Umar, M.; Kurnadi, B.; Rianto, E.; Pangestu, E.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.159-166

Abstract

This study was aimed to evaluate the effect of dietary energy level on daily gain of Madura cattle and their blood metabolites (glucose and blood urea). Twelve (12) male Madura cattle aged at 2 years old, 156.27+6.92 kg (CV 4.43%) of body weight were used in this study which was assigned to completely randomized design for three feeding treatments (low, middle and high energy contents) and four replications. The daily gain was measured for 90 days, while the blood metabolites were measured at the middle of experiment. Results showed that dietary energy levels did not affect (P>0.05) average daily gain, dry matter intake, dry matter digestibility, energy intake and feed conversion ratio. Blood glucose and blood urea concentration were ranged at 67-75 and 35-50 mg/dL, respectively, while feed energy efficiency reached 0.145 MJ/g ADG. In conclusion, high-level energy could not provide better performance in Madura cattle because of a limitation factor on the DMI.
FEED POTENTIAL OF AGRICULTURE WASTE FOR BEEF CATTLE DEVELOPMENT IN KUNINGAN REGENCY, WEST JAVA Farda, F. T.; Laconi, E. B.; Mulatsih, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.167-175

Abstract

The aims of research were to identify and analyze potential agricultural waste used as feed, examines the characteristics of beef cattle ranchers and estimate the ability of the addition of beef cattle population in Kuningan Regency. The primary data were taken from interviews with 30 respondents beef cattle farmers selected by purposive sampling in three districts based on the largest beef cattle population as a recommendation by local government of Kuningan Regency Agricultural. Waste samples taken randomly three times to analyze of nutrient composition by proksimat analyze was the type of the most widely used for feed. Secondary data was obtained from Kuningan Regency Veterinary Office, Department of Food Crops and the Central Statistics Agency. The results showed that the type of agricultural waste used in Kuningan Regency from highest to lowest production is rice straw, hay sweet potatoes, peanuts and hay with traditional animal husbandry systems. Districts that can improve beef cattle population from the highest to lowest number was Luragung, Cibingbin, Ciwaru, Subang, Maleber, Cibeureum, Cilebak, Karangkancana and Cimahi. In conclusion, the highest agricultural waste production was rice straw and the highest potential for the development of beef cattle in the Kuningan Regency was Luragung District.

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