Journal of the Indonesian Tropical Animal Agriculture
Vol 38, No 4 (2013): (December)

GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

Putra, W.P.B. (Unknown)
Hartatik, T. (Unknown)
Sumadi, S. (Unknown)

Article Info

Publish Date
01 Dec 2013

Abstract

The objective of this research was to identify growth hormone (GH) genes genotype in selectedAceh cattle at Indrapuri’s Breeding and Forage Centre (IBFC) of Aceh Cattle. Fourty one cattleconsisting of 21 male and 20 female cattle were used in this study. The genomic DNA was extractedfrom blood using Sambrook et al. (1989) methods. Polymerase Chain Reaction - Restriction FragmentLength Polymorphism (PCR-RFLP) and mehod of sequencing was used to detect MspI site on GH gene.Based on sequencing results, it can be concluded that all cattle were monomorphism. The frequency ofTT genotype were 1.00 and same as T allele frequency. The transition of C (cytosine) into T (thymine)on 1548 bp caused the lost of restriction site.

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Journal Info
Journal of the Indonesian Tropical Animal Agriculture
Website

Abbrev

jitaa

Publisher

Universitas Diponegoro

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research ...