Biotropika
Vol 2, No 1 (2014)

KUALITAS SEMEN SEGAR KAMBING BOER PADA TEMPERATUR PENYIMPANAN 4 OC DENGAN MENGGUNAKAN PENGENCER SITRAT DAN SUPLEMENTASI SUSU KEDELAI BUBUK

Rahayu, Winda (Unknown)
W.M, Agung Pramana (Unknown)



Article Info

Publish Date
16 Jul 2014

Abstract

ABSTRACT The objective of this research was to know the influence of soybean milk powder diluents based on concentration (2%, 4%,6%) in citrate diluent to motility, viability, abnormality and membrane integrity of spermatozoa based on long duration storage (0 hours, 24 hours, 72 hours, 120 hours). This research using diluent medium based on vegetable medium (lechitin) is andromed (K+), soybean milk in diluent citrate with concentrations of 0% (K-), 2% (F1), 4% (F2), and 6% (F3). The characterization of predilution were observed such as volume, color, consistency, pH, mass motility, individual motility, viability, concentration, and abnormality. Next step dilution based on treatment with each of the three  recurrence and performed storage at temperature of 4˚C. The next of post storage were observed such as sperm viability, motility, abnormality and membrane integrity. Data were analyzed using two way ANOVA in factorial completely randomized design with SPSS version 16.0 and continued with Tukey test. The results showed that a significant difference (P<0.05)  to K+, F1, and F2 diluent to K- and F3 diluent. The presence of lechitin content of soybean milk can protect spermatozoa from cold shock during storage at a temperature of 4˚C. The concentration of soy milk powder high possibility changes to pH of the media and influenced of osmotic presure on sperm membrane decrease of the sperm quality during storage. Key word: Lechitin, plasma membrane, soybean milk

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Journal Info

Abbrev

biotropika

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, ...