agriTECH
Vol 29, No 4 (2009)

A Simple and Sensitive Method for Determination of Sugar Content in Fruits and Culture Media During Fermentation

Djagal Wiseso Marseno (Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Flora, Bulaksumur, Yogyakarta 55281)
Retno Indrati (Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Flora, Bulaksumur, Yogyakarta 55281)

Article Info

Publish Date
15 May 2012

Abstract

A colorimetric for sugar determination using cuprammonium reagent containing strong base has been improved with a heating process after a reaction started.  The reaction was started after the sugar or sample containing sugar was added to the cuprammonium reagent consisted of 1 mM CuSO – 0,4 M (NH ) SO – 0,6 M NaOH).  This heat­ ing process could enhance the formation of sugar­cupper ammonium complex, so that the intensity of absorbance increased at wavelength of 280 nm. This simple and sensitive method was applicable for various sugars detection such as sugar fraction after HPLC separation, sugar content in biological extract or in culture medium during fermentation. Interestingly, different from previous report, this method was specific for non polyol sugars.

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Journal Info
agriTECH
Website

Abbrev

agritech

Publisher

Universitas Gadjah Mada

Subject

Agriculture, Biological Sciences & Forestry

Description

Agritech with registered number ISSN 0216-0455 (print) and ISSN 2527-3825 (online) is a scientific journal that publishes the results of research in the field of food and agricultural product technology, agricultural and bio-system engineering, and agroindustrial technology. This journal is ...