Anemiasufferers in Indonesia are increasing. Anemia diagnosisconfirmed by examining red blood cell (RBC) which requires Hayem as reagent. Problemof running out reagents still widely found that distrupts health services.0.9% infusion fluid is physiological solution and readily available. Therefore, this study aims to ultilize 0.9% intravenous fluids as alternative to Hayem in RBC examination by analyzing differences in results of alternative reagents to Hayem reagent. This type of research is experimental, using normality test, t-test, and linear regression.Carried out in the hematology laboratory of Nahdlatul Ulama University Surabaya in February 2020.Use 25 samples from students and health faculty staff with average infusion fluid ie 4,54 x 106 /µL (SD 0,59 x 106 /µL) while Hayem reagent ie 4,63 x 106 /µL (SD 0,52 x 106 /µL)and there is no difference two results (P = 0.197). fluid normality test was obtained Sig.= 0,716, while Hayem reagent Sig.= 0,923. Paired sample t-test obtained Sig.= 0,197. Resultsof linear regression test showed correlation coefficient of 0.823 and coefficient determination of 67.8% (P = 0.000) with equation Y = 0.725X + 1.339. It was concluded that 0.9% infusion fluid could be used as alternative reagent for Hayem in RBC examination.
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