The purpose of this research is to know the existence of mercury metal degradation bacteria in the activity of illegal gold mining (PETI) in Simpi Village, Belitang Hilir sub-district, Sekadau District, West Kalimantan. The samples were taken from one location with three different sampling points. Isolation of bacteria by pour plate method in NA-HgCl medium. Detection of bacteria by paper disc method based on inhibit zone resistant of Mueller-Hinton Agar (MHA) with mercury levels such as 10 mg/L, 20 mg/L and 30 mg/L . The results showed that it was found that one pure isolate (PP) showed the most resistant isolate to the mercury stress of 10 mg/L can be detected
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