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Indonesian Journal of Pure and Applied Chemistry

IJoPAC
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Published by Universitas Tanjungpura

ISSN : 26208571 EISSN : 2620858X DOI : -

Core Subject : Science,

Biochemistry, Genetics & Molecular Biology Chemistry

Indonesian Journal of Pure and Applied Chemistry (IJoPAC) was first established in 2018 as a scientific journal that accommodates researchers and education practitioners to publish scientific work and research results which are the results of experiments or literature studies in the field of chemistry. Publications are carried out periodically with three issues (January-April, May-August, September-December) in one volume per year. Published scientific work can be in the form of original articles, short communications and review results covering the fields of Physical chemistry, Organic chemistry, Analytical chemistry, Inorganic chemistry, Biochemistry / Biological Chemistry, Applied Chemistry. Focus and Scope of Indonesian Journal of Pure and Applied Chemistry covering the fields : Physical chemistry Organic chemistry Analytical chemistry Inorganic chemistry Biochemistry/Biological Chemistry Applied Chemistry

Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)

Articles 76 Documents

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AMPLIFIKASI PCR DOMAIN D1/D2 28S rDNA MENGGUNAKAN PRIMER ITS1 DAN ITS4 SAMPEL DNA DARI Candida tropicalis YANG DIISOLASI DENGAN METODE PENDINGINAN Hermansyah Hermansyah; Novian Sutami; Miksusanti Miksusanti
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 1 (2018)
Publisher : Tanjungpura University

The purpose of this research was to isolated DNA from the yeast C. tropicalis with freeze thawing method -200 C conducted on 3 colonies of C. tropicalis. Each colony threated variations of cooling, 3x15 minutes, 3x25 minutes and 3x35 minutes, to break the cell walls. Subsequently all the samples amplified with 3 variations of PCR cycles, 15 cycles, 25 cycles and 35 cycles, after all of the samples isolated by freeze thawing method -200 C. Its was known that sample A15 has the smallest concentration of DNA yeast C. tropicalis, ie 50 µg/mL, while sample C35 had the largest concentration of DNA yeast C. tropicalis, ie 225 µg/mL. The result of the research indicated that the best condition can be reached in 3x35 minutes. On 35th cycle has clearer C. tropicalis DNA bands than the 25th and 15th PCR cycle. C. tropicalis DNA bands at 35th cycles there were 7 DNA bands were detected and bright bands on a long 35 minutes cooling. In the 25th and the 15th cycle, there was no DNA bands were detected in all samples. Based on the results obtained, the amplification process must be carried out at least 35 times cycles so that the C. tropicalis DNA bands can be detected.

SINTESIS DAN KARAKTERISASI SILIKA GEL DARI LIMBAH KACA SERTA APLIKASINYA PADA KROMATOGRAFI KOLOM Verry Andre Fabiani; Nelly Wahyuni; Ridho Brilliantoro; Mellisa Nurul Safitri
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 1 (2018)
Publisher : Tanjungpura University

Silica gel synthesis from glass waste have been carried out with activation and purification method by using hydrochloride acid. Hydrochloride acid were varied 8 N, 10 N, and 12 N to observe optimum concentration in purification of silica gel and component separation in chromatography column. Gravimetric analysis result showed 12 N hydrochloride acid produce ~100 % silica. XRD and FTIR analysis revealed that synthetic gel silica was amorphous where its IR absorption bands were at 3426 cm-1, 1620 cm-1, 1080 cm-1, 772 cm-1 and 478 cm-1. The absorption bands agreed to standard gel silica and spectrum. The most effective HCl in gel silica synthesis was 12 N, caused by its ability to separate color component in a column. The synthetic silica that can be applied as stationary phase in column chromatography.

PENINGKATAN KUALITAS MINYAK JELANTA MENGGUNAKAN KARBON AKTIF DAN EKSTRAK PUCUK IDAT (Cratoxylum glaucum) Robby Gus Mahardika; Sito Enggiwanto; Ary Samsiar
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 1 (2018)
Publisher : Tanjungpura University

Silica Waste cooking oil can be used as a soap or biodiesel. Good soaps or biodiesel should be from oils that have low levels of fatty acids and free radicals. However, waste cooking oil has high free fatty acid and free radical, it is necessary to increase the quality of waste cooking oil. One effort to improve the quality of waste cooking oil can use activated carbon as an adsorbent. Decrease in free radicals in cooking oil can use antioxidants from extract pucuk idat (Cratoxylum glaucum). This study aims to see the effect of extract pucuk idat on the process of improving the quality of waste cooking oil. The process of improvement by adding activated carbon and varying the concentration of ethanol extract pucuk idat. Activated carbon used 10% with variation of extract 0,25%; 0,5% and 0,75%. This process followed by stirring for 15 minutes at 80°C, then soaked for 3 days. Oil quality are identified by the method of determining the levels of free fatty acids and acid numbers. The results of this study indicate that extract pucuk idat in ethanol with 0,75% concentration has the lowest free fatty acid and acid number. Extract pucuk idat can improve the quality of waste cooking oil.

KINETIKA DEGRADASI SELULOSA ASETAT DARI SABUT PINANG Intan Syahbanu; Annisa Anugraini; Husna Amalya Melati
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 1 (2018)
Publisher : Tanjungpura University

Cellulose Acetate is a material with wide range in applications. Cellulose acetate may obtain from natural resources, such as areca fiber. Polymers should have uniform molecular weight for the applications and could be modify by ultrasonic waves. Ultrasonic treatment on polymer entangled with polymer degradation. In this research, degradation kinetics of cellulose acetate in acetone had been studied. Analysis was performed by determination of intrinsic viscosity in polymer solution system. Intrinsic viscosity was successfully obtained by fitting data using Huggins equation, indicate molecular interaction in polymer solution should not be neglected. Increasing in sonication time reducing the intrinsic viscosities as well as molecular size in solutions. Degradation rate constant and reaction order were obtained 5,975x10-3(g.mL-1)0,1.min-1 and 1,0616, respectively. Kinetics model was successful to determine the degradation rates of cellulose acetate on acetone by correlation coefficient (R2) at 0,966.

EKSTRAKSI DAN PENENTUAN GUGUS FUNGSI ASAM HUMAT DARI PUPUK KOTORAN SAPI Nurlina Nurlina; Intan Syahbanu; Mirna Tersiana Tamnasi; Chyntia Nabela; Maria Desi Furnata
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 1 (2018)
Publisher : Tanjungpura University

Cow manure fertilizer was used as raw material for the extraction of humic acid in this research. The aim of this research were extraction of humic acid from cow manure fertilizer, characterization of functional groups based on FTIR spectra, and determination of humic acid functional groups. Humic acid extraction from cow manure used 0.050 M; 0.100 M; 0.250 M dan 0.500 M KOH solution. A cow manure fertilizer were shaken with KOH solution for 1; 2; 4 and 6 h. The quantitative determination of oxygen-containing functional groups of humic acid was conducted by titrimetri. The FTIR spectrum showed the presence of –OH group and N-H stretching (bands 3361.46 cm-1); C-H aliphatic for CH2, CH3 (2946.52 cm-1 and 1442.24 cm-1); C=C aromatic components (1626.48 cm-1); COO- symmetric stretching (1521.71 cm-1); C-O stretching from –COOH (1229.74 cm-1). Total acidity value was 500 cmole.kg-1 (Baryta Absorption Method); carboxyl groups content was 250 cmole.kg-1 (Ca-Acetate Method); and phenol (OH) concentration was 250 cmole.kg-1. It can be concluded that humic acid successfully extracted from cow manure fertilizer using 0.250 KOH solution as extractant for 2 h, which has yield of 4.486% humic acid.

ANALISIS UKURAN PARTIKEL SILIKA HASIL EKSTRAKSI DARI BATU PADAS ASAL KABUPATEN KETAPANG KALIMANTAN BARAT Risya Sasri; Nurlina Nurlina; Lia Destiarti; Intan Syahbanu
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 1 (2018)
Publisher : Tanjungpura University

Analysis of particle size of silica was extracted from rocks from Ketapang Regency, West Kalimantan based on characterization using scanning electron microscope (SEM) have been carried out. The analysis was performed to determine the particle size change in extraction silica without and with activation using acid solution. SEM image processing was performed on a 10 μm scale at 350x magnification through the image-J software to determine the particle size distribution. The results of the analysis showed that the area and diameter of the silica particle extracted from rocks were 3,935 μm2 and 3,147 μm (without activation) and 4,28 μm2 and 3,267 μm (activated) respectively.

ANTIFUNGAL ACTIVITY OF METHANOL EXTRACT OF BLACK CUMIN SEEDS ON Aspergillus sp. Lia Utami Rahman
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 2 (2018)
Publisher : Tanjungpura University

Aspergillus sp. is a type of pathogenic fungus and can cause pulmonary aspergillosis. This research aims to determine the concentration of methanol extract from black cumin (Nigella sativa) seeds which effective to inhibit the growth of Aspergillus sp. (RMPRB2). This research used poisoned food method with Completely Randomized Design consisting of six treatments with four replications. The results showed that the methanol extract of Nigella sativa seeds could inhibit the growth of fungal isolates from Aspergillus sp. (RMPRB2). The Methanol extract of Nigella sativa with a concentration of 2% and the positive control gave a strong inhibitory activity to the growth of Aspergillus sp. (RMPRB2) with a percentage of 100%. Concentration of 1% is the most effective result because gave a very strong inhibitory activity to the growth of fungal isolates of Aspergillus sp. (RMPRB2).

EKSTRAKSI DAUN PELAWAN (Tristaniopsis merguensis)SEBAGAI ANTIOKSIDAN MENGGUNAKAN MICROWAVE ASSISTED EXTRACTION (MAE) Sito Enggiwanto; Fusfita Istiqomah; Kasro Daniati; Occa Roanisca; Robby Gus Mahardika
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 2 (2018)
Publisher : Tanjungpura University

Indonesia is a country that produces a wealth of abundant biodiversity, one of which is a plant that is often cultivated the tree of Pelawan (Tristaniopsis merguensis). Pelawan trees are widely spread in Indonesia one of them found in the forests of the Bangka Belitung island. Therefore, this study aims to identify the secondary metabolites and testing antioxidants in leaf of pelawan extract by comparing methods of Microwave Assisted Extraction (MAE) with maceration method. The resistant leaf used in this study was obtained from Sempan, Bangka Belitung. The observation result shows that the use of MAE method can extract with faster time compared with maceration method. So the MAE method is more effective than the maceration method. The using of MAE method for 30 minutes obtained the highest yield percentage of 41.3% in ethanol solvent which is more effective than acetone solvent. Extraction by using 48 hour maceration yields is 28.985%. While the extraction with 30 minute maceration is 13.385%. Testing of antioxidant activity using Microwave Assisted Extraction (MAE) method on acetone solvent has the highest antioxidant activity than ethanol extract that is 9,501 μg/mL. The use of the MAE method is more efficient in extracting the leaves of Pelawan than the maceration method when viewed from the time and antioxidant activity.

DETEKSI KEBERADAAN BAKTERI RESISTEN LOGAM MERKURI (Hg) PADA PENAMBANGAN EMAS TANPA IZIN (PETI) DI SIMPI, SEKADAU, KALIMANTAN BARAT Abdullah Abdullah
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 2 (2018)
Publisher : Tanjungpura University

The purpose of this research is to know the existence of mercury metal degradation bacteria in the activity of illegal gold mining (PETI) in Simpi Village, Belitang Hilir sub-district, Sekadau District, West Kalimantan. The samples were taken from one location with three different sampling points. Isolation of bacteria by pour plate method in NA-HgCl medium. Detection of bacteria by paper disc method based on inhibit zone resistant of Mueller-Hinton Agar (MHA) with mercury levels such as 10 mg/L, 20 mg/L and 30 mg/L . The results showed that it was found that one pure isolate (PP) showed the most resistant isolate to the mercury stress of 10 mg/L can be detected

PHYTOCHEMICAL SCREENING, ANTIOXIDANT, AND CYTOTOXICITY OF Zamioculcas zamiifolia ROOT EXTRACT Rini Muharini; Masriani Masriani; Rudiyansyah Rudiyansyah
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 2 (2018)
Publisher : Tanjungpura University

Zamioculcas zamiifolia is an ornamental plant that had been used pharmacologycally as a traditional medicine plant. The aims of this research were to investigate the phytochemistry of n-hexane, chloroform and methanol extracts from the roots of Z. zamiifolia and to evaluate their biological activities as antioxidant and cytotoxic. All extracts were screened phytochemically using specific reagents and TLC technique to reveal the major component of each extract. The chloroform was obtained as the highest quantity of extract which gave positive test for steroids and flavonoids. All extracts were tested for their antioxidant activity using DPPH method and for their cytotoxic activity against two types of human cancer cell lines, HepG2 and T47D, and normal African green monkey kidney ephitelial cell lines, Vero. The methanol and chloroform extract exhibited antioxidant activity with AA50 values of 180.0 and 431.5 µg/mL, respectively. Chloroform and methanol extracts showed potencial and moderate cytotoxicity towards T47D cell line with IC50 433.1 and 461.1µg/mL, respectively. None of the extracts was toxic against HepG2 and Vero cell lines.

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Home Page

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Editorial Address Department of Chemistry, Faculty of Mathematics and Natural Sciences, Tanjungpura University Jl. Prof. Dr. Hadari Nawawi, Pontianak, Kalimantan Barat, Indonesia

Location Kota pontianak, Kalimantan barat INDONESIA

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Contact Name
Ajuk Sapar

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ajuk.sapar@chemistry.untan.ac.id

Phone
+6281282990575

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anthoni.b.aritonang@chemistry.untan.ac.id

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Department of Chemistry, Faculty of Mathematics and Natural Sciences, Tanjungpura University Jl. Prof. Dr. Hadari Nawawi, Pontianak, Kalimantan Barat, Indonesia

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Kota pontianak,

Kalimantan barat

INDONESIA