Indonesian Journal of Biotechnology
Vol 26, No 1 (2021)

Reaction kinetics of lactic acid fermentation from bitter cassava (Manihot glaziovii) starch by Lactobacillus casei

Setiyo Gunawan (Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Jl. Teknik Kimia, Keputih, Kota Surabaya, Jawa Timur 60111)
Nurul Rahmawati (Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Jl. Teknik Kimia, Keputih, Kota Surabaya, Jawa Timur 60111)
Rona Bening Larasati (Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Jl. Teknik Kimia, Keputih, Kota Surabaya, Jawa Timur 60111)
Ira Dwitasari (Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Jl. Teknik Kimia, Keputih, Kota Surabaya, Jawa Timur 60111)
Hakun Wirawasista Aparamarta (Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Jl. Teknik Kimia, Keputih, Kota Surabaya, Jawa Timur 60111)
Tri Widjaja (Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Jl. Teknik Kimia, Keputih, Kota Surabaya, Jawa Timur 60111)



Article Info

Publish Date
30 Mar 2021

Abstract

One of the utilizations of bitter cassava is modified cassava flour (Mocaf) production using the fermentation process by Lactobacillus casei. The Mocaf has potential as the future of food security products. It has a characteristic property similar to wheat flour. Lactic acid was also produced as a by‐product during fermentation. After 40 h of fermentation, the proximate composition content of Mocaf was lactic acid content of 0.000928 g/L, hydrogen cyanide levels of 0.02 ppm, starch content of 59.13%, amylose content of 12.98% and amylopectin content of 46.15%. In the scaling‐up process from a laboratory scale to a pilot and industrial scale, modeling is needed. There are five equation models used to describe the kinetic reactions of lactic acid from bitter cassava starch: Monod, Moser, Powell, Blackman, and Product Inhibitor. Each parameter was being searched by a fitting curve using sigmaplot 12.0. The best result in terms of the highest R2 (0.65913) was obtained in the Powell equation with the value of µmax of 1.668/h, Ks of 123.4 g/L, and maintenance rate (m) of 4.672. The kinetic data obtained can be used to design biochemical reactors for industrial scale Mocaf flour production.

Copyrights © 2021






Journal Info

Abbrev

ijbiotech

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...