This research aims to understand the effect of dosages combination ofmixed hCG and hypophysis extract injection toward volume and quality of semenof Rasbora lateristriata Blkr. There were 5 treatments applied, namely PA=control (1 ml of Na Cl 0,65 % /kg body weight), PB = hCG 400 IU/kg bodyweight + CPE 2 dosages, PC = hCG 600 IU/kg body weight + CPE 2 dosages,PD = hCG 800 IU/kg body weight + CPE 2 dosages, PE = hCG 1000 IU/kgbody weight + CPE 2 dosages, PF = hCG 1200 IU/kg body weight + CPE 2dosages. The best result for male fish was the combination of hCG 1000 IU/kgbody weight + CPE 2 dosages, the semen volume was 0.87 ml, spermconcentration was 18,43 x 10 9/ml, sperm viability was 88.67% and spermmotility was 86.00%
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