Biota
Vol 13 No 2 (2020)

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

Ririn Puspadewi (Universitas Jenderal Achmad Yani)
Putranti Adirestuti (Universitas Jenderal Achmad Yani, Cimahi, West Java, Indonesia)
Mira Andam Dewi (Universitas Jenderal Achmad Yani, Cimahi, West Java, Indonesia)
Wakilatul Hasanah (Universitas Jenderal Achmad Yani, Cimahi, West Java, Indonesia)



Article Info

Publish Date
31 Dec 2020

Abstract

Candida spp secreted a different kind of extracellular enzymes. Protease and lipase are the enzymes that are commonly used in the food and pharmaceutical industries. This study aimed to examine protease and lipase activity of Candida tropicalis isolated from the soil of the Medicinal Plant Garden of the Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Indonesia. Candida isolate was subjected to the fermentation process to obtain bioactive metabolites. The result was analyzed using ANOVA within a 5% interval of confidence, continuing with PostHoc. The result showed the Candida tropicalis metabolite giving the best proteolytic index value (0,6556 ± 0,0090) U/mL. The metabolite isolate of Candida tropicalis had the highest activity, amounting to 5,776 ± 0,495 U/mL. The best results of lipolytic index value (0,394 ± 0,053) U/mL. The Candida tropicalis metabolites produced the highest lipase enzyme after nine days of fermentation in 5.2917±0,0167 U/mL.

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Journal Info

Abbrev

jb

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology

Description

Focus and Scope of Biota on Bioscience, includes: cell biology and genetics, biodiversity, ecology, physiology and biotechnology. Biota covers all life forms, including microbes, fungi, plants, virus, animal and human. Focus and Scope of Biota on Biology Education, includes: Experimental Research, ...