Bioscientia Medicina : Journal of Biomedicine and Translational Research
Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research

Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline

Rachmat Hidayat (Department of Biology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia)
Patricia Wulandari (Cattleya Mental Health Center, Palembang, Indonesia)

Article Info

Publish Date
29 Jan 2021

Abstract

ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.

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Journal Info
Bioscientia Medicina : Journal of Biomedicine and Translational Research
Website

Abbrev

bsm

Publisher

Universitas Sriwijaya

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

Description

BioScientia Medicina is an open access international scholarly journal in the field of biomedicine and translational research aimed to publish a high-quality scientific paper including original research papers, reviews, short communication, and technical notes. This journal welcomes the submission ...