Indonesian Journal of Tropical Aquatic
Vol. 4 No. 1 (2021): February

Analysis of Vannamei shrimp DNA fragment resistant to White Spot Virus Syndrome

Wiwien Mukti Andriyani (Balai Budidaya Air Payau Situbondo)
Sumini Sumini (Balai Budidaya Air Payau Situbondo, Indonesia)
Zeny Widiastuti (Balai Budidaya Air Payau Situbondo, Indonesia)



Article Info

Publish Date
01 Mar 2021

Abstract

The attack of WSSV in Vannamei shrimp cultivation is still common. Shrimp quality improvement can be made through selection with the help of markers (marker-assisted choice). This study aimed to evaluate the DNA fragment profile of white shrimp that was resistant to WSSV disease. The analysis was performed using the PCR-RAPD method. WSSV challenged four groups of 100 Vannamei shrimp, then DNA was extracted from live and dead shrimp. The results showed that 2 of the 17 primers tested had high potential as markers, namely OP-09 and OPD-2. PCR products with OPC-09 primers had specific DNA bands measuring about 1.2 kb in all post-challenge WSSV resistant shrimp individuals. The amplification results using OPD-02 primers showed a particular band of DNA with a length of about 1.0 kb, with 60 % of the appearance in WSSV-resistant shrimp. In contrast, the WSSV-susceptible shrimp group did not have specific DNA fragments. Thus, the two RAPD primers had a high chance of being used in the selection with the help of markers to produce WSSV resistant shrimp.

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Journal Info

Abbrev

ijota

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Indonesian Journal of Tropical Aquatic (IJOTA) is open access publishes papers two times a year in February and Augst. IJOTA will publish the research results useful for the sustainability of fisheries. IJOTA is a scientific journal that discusses the results of research in science, technology, ...