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All Journal Indonesian Journal of Tropical Aquatic
Zeny Widiastuti
Balai Budidaya Air Payau Situbondo, Indonesia
Agriculture, Biological Sciences & Forestry

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Analysis of Vannamei shrimp DNA fragment resistant to White Spot Virus Syndrome Wiwien Mukti Andriyani; Sumini Sumini; Zeny Widiastuti
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 4 No. 1 (2021): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v4i1.15839

Abstract

The attack of WSSV in Vannamei shrimp cultivation is still common. Shrimp quality improvement can be made through selection with the help of markers (marker-assisted choice). This study aimed to evaluate the DNA fragment profile of white shrimp that was resistant to WSSV disease. The analysis was performed using the PCR-RAPD method. WSSV challenged four groups of 100 Vannamei shrimp, then DNA was extracted from live and dead shrimp. The results showed that 2 of the 17 primers tested had high potential as markers, namely OP-09 and OPD-2. PCR products with OPC-09 primers had specific DNA bands measuring about 1.2 kb in all post-challenge WSSV resistant shrimp individuals. The amplification results using OPD-02 primers showed a particular band of DNA with a length of about 1.0 kb, with 60 % of the appearance in WSSV-resistant shrimp. In contrast, the WSSV-susceptible shrimp group did not have specific DNA fragments. Thus, the two RAPD primers had a high chance of being used in the selection with the help of markers to produce WSSV resistant shrimp.
Co-Authors Sumini Sumini Wiwien Mukti Andriyani