Agrivet
Vol 18, No 1 (2012): AGRIVET

Induction of “Sakuntala” chrysanthemum bud using in-vitro culture

Tutut Wirawati (Universitas Pembangunan Nasional "Veteran" Yogyakarta)
Ari Wijayani (Universitas Pembangunan Nasional "Veteran" Yogyakarta)
Rina Srilestari (Universitas Pembangunan Nasional "Veteran" Yogyakarta)



Article Info

Publish Date
30 Jun 2012

Abstract

The research was to determine the best combination of Chrysanthemum explant and concentrations of BAP (6 - benzyl amino purine), the effect of the explant material and the effect concentrations of BAP (6 - benzyl amino purine) in vitro culture by Murashige and Skoog medium. The experimental methode was Completely Randomizes Design (CRD) which consisted of two factors. The first factor is the explant material, consisting of three standards are: P1 = node, P2 = shoots, P3 = leaf. The second factor is the concentration of BAP which consists of four levels, namely: B1 = 0.5 ppm, 1.0 ppm = B2, B3 = 1.5 ppm, B4 = 2.0 ppm. The results showed there were significant interaction on fresh weight, dry weight, number of shoots and length of shoots. The treatment combination between bud explants with BAP 0.5 ppm, showed the best results on fresh weight, number of shoots and long shoots. Use of explant shoots, can accelerate the current growth of shoots, expanding the number and length of root buds. The addition of plant growth regulators BAP 0.5 ppm in the tissue culture medium, can spur the rapid growth of shoots, root number and length of root best.Key words : in vitro, chrysanthemum, kinds of eksplant, the concentration of BAP

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Journal Info

Abbrev

agrivet

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Agrivet (ISSN: 1410-3796) adalah jurnal ilmiah yang mempublikasikan hasil penelitian dan ulasan mengenai berbagai aspek yang terkait dengan Agronomi dan bidang pertanian yang terkait (Budidaya Tanaman, Pemuliaan Tanaman, Hama Penyakit Tanaman dan Sumber Daya Lahan). Agrivet diterbitkan oleh Prodi ...