Chemistry Indonesian Journal of Chemical Research
Vol 9 No 2 (2021): Edition for September 2021

Qualitative Protein Hydrolyzed from Nerita undata in Supralittoral Rocks and Mezolittoral Zone of Hasa Cape Using TLC

Healthy Kainama (Department Chemistry Education, Universitas Pattimura, Ambon, Indonesia)
Hanoch J. Sohilait (Department Chemistry, Universitas Pattimura, Ambon, Indonesia)
Christian Jacob Souisa (Centre For Deep-Sea Research, Indonesian Institute of Sciences, Ambon, Indonesia)



Article Info

Publish Date
30 Sep 2021

Abstract

Gastropods are the sources of protein for coastal communities in the Maluku islands. We conducted analysis quantitative and qualitative of protein in Nerita undata meat from Hasa Cape in Saparua Island. The percentage of protein was analyzed by the Kjeldahl method. Qualitative analysis began with breaking peptide bonds in protein to amino acid components by sulphuric acid and barium hydroxide hydrolyzed. We identified amino acid compounds by using thin-layer chromatography (TLC) in butanol-acetic acid-water (8:1:1, v/v) as eluent. The result showed that N. undata meat contains 11.15% of protein and twelve amino acid compounds. There are seven essential amino acids in N. undata meat from supralittoral rocks and mezolittoral zone of Hasa cape is a source of quality protein. Thus, this species can be considered as a source of high-quality protein.

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Journal Info

Abbrev

Indonesian Journal of Chemical Research (Indones.

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Engineering Environmental Science

Description

Indo. J. Chem. Res. is a journal that regularly publishes primary articles covering all branches of chemistry and its sub-disciplines. Published articles consist of complete research, short communication, and review articles. The scope of published articles is not limited to inorganic chemistry, ...