Folia Medica Indonesiana
Vol. 57 No. 3 (2021): September

Phylogenetic and Transmission Patterns of Extended-Spectrum β- Lactamase –Producing Escherichia Coli among Neonatal’s Gut Flora in Indonesia

Rakhmatul Binti Sulistya (Master Program in Basic Medical Sciences, Faculty of Medicine, Universitas Airlangga, Surabaya)
Kuntaman Kuntaman (Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya/ Dr. Soetomo General Academic Hospital, Surabaya)
Eko Budi Koendhori (Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya/ Dr. Soetomo General Academic Hospital, Surabaya)
Terza Aflika Happy (Postgraduate Program of Tropical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya)
Wahyu Setyarini (Institute of Tropical Disease, Universitas Airlangga, Surabaya)



Article Info

Publish Date
05 Sep 2021

Abstract

This study aimed to explore the phylogenetic pattern of Extended-spectrum β-lactamase-producing Escherichia coli in the gut flora of neonatal into groups A, B1, B2, D and tracing the presence of spreading among the age group of neonatal. Multiplex PCR was conducted to classify the phylogenetic group of ESBL producing E. coli into groups of commensals (A, B1) and pathogen (B2, D). The spread of bacterial agents among the host (neonatal) was conducted by the RAPD-PCR method with two random primers. Among 34 bacterial isolates were identified among early neonatal group A (22,2%), B1 (11,1%), B2 (22,2%) D (33,3%) and postneonatal groups A (8%), B1 (4%), B2 (36%) D (40%). It was not a statistically significant difference (p=0.388). The dominance of pathogenic groups B2 and D as many as 70,6%. From 34 samples was found 34 types of RAPD indicated there were not any bacterial spread among neonatal. The ESBL producing E. coli among early neonatal and postneonatal were dominated by the pathogenic group of B2 and D (total=70,6%), but they were not statistically significant. There was not any spreading of bacterial agents among individual gut flora of neonatal.

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Journal Info

Abbrev

FMI

Publisher

Subject

Other

Description

Folia Medica Indonesiana publishes articles in the field of medical science in particular and health sciences in general, as well as a variety of other fields related to those two disciplines. Most of the articles are research article, and others are articles on case reports and literature review. ...