SAINS TANAH - Journal of Soil Science and Agroclimatology
Vol 18, No 2 (2021): December

Molecular identification of isolates from local microorganisms as potential biofertilizer

Abdul Hasyim Sodiq (Agroecotechnology Department, Faculty of Agriculture, Sultan Ageng Tirtayasa University)
Mieke Rochimi Setiawati (Faculty of Agriculture, Padjadjaran University)
Dwi Andreas Santosa (Department of Soil Science and Land Resources, Facuty of Agriculture, IPB University)
Dedi Widayat (Faculty of Agriculture, Padjadjaran University)



Article Info

Publish Date
31 Dec 2021

Abstract

Local microorganisms (MOL) are liquid fertilizers commonly used by farmers to help increase crop production. Beneficial microbes in MOL need to characterize their interactions and ability to produce growth drive compounds. The purpose of this research is to identify the superior microbial isolates from MOL made by farmers from Cibodas Lembang Bandung, Indonesia that can produce phytohormones as biofertilizers. The results of the microbial selection of MOL derived from three best microbes are 1A-2 NFB, 4A-1 NFB, and 4B-1 NFB with the ability to produce auxin, i.e., 19.41 ppm, 17.18 ppm, and 10.59 ppm, respectively. The compatibility test between the three isolates showed negative results so that it was possible to apply three microbes as a consortium. The results of a molecular identification with a 16S rRNA analysis indicate strain microbe 1A-2 NFB: Bacillus cereus (99.88% homology), 4A-1 NFB: Bacillus cereus (99.76% homology), and 4B-1 NFB: Lysinibacillus sp. (99.88% homology).

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