Indonesian Journal of Chemistry
Vol 21, No 6 (2021)

The Biotransformation and Biodecolorization of Methylene Blue by Xenobiotic Bacterium Ralstonia pickettii

Adi Setyo Purnomo (Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia)
Asranudin Asranudin (Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia)
Didik Prasetyoko (Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia)
Yulinar Dwi Nur Azizah (Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia)

Article Info

Publish Date
12 Oct 2021

Abstract

The biotransformation and biodecolorization of methylene blue (MB) dye using the bacterium Ralstonia pickettii was investigated. This experiment was conducted in a nutrient broth (NB) medium after adding MB at 100 mg L–1 concentration. Approximately 98.11% of MB was decolorized after 18 h of incubation. In addition, the metabolic products detected by LC-TOF/MS were Azure A (AA), thionine, leuco-MB, and glucose-MB, which indicated the MB degradation through a reductase that attacked the heterocyclic central chromophore group present in the structure. Moreover, azure A and thionine fragments resulted from the N-demethylase enzyme that attacked the auxochrome group. Thus, this research was assumed to be the first scientific report suggesting the potential to use R. pickettii in the biodecolorization and biotransformation of dye waste, particularly MB.

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Journal Info
Indonesian Journal of Chemistry
Website

Abbrev

ijc

Publisher

Universitas Gadjah Mada

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology ...