Biosaintifika: Journal of Biology & Biology Education
Vol 10, No 3 (2018): December 2018

Purification and Partial Characterization of α-Amylase Produced by a Thermo-Halophilic Bacterium Isolate PLS 75

Teuku M. Iqbalsyah (Biomolecules Application Research Group, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala)
Farah Fajarna (Biomolecules Application Research Group, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala)
Febriani Febriani (Biomolecules Application Research Group, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala)



Article Info

Publish Date
06 Dec 2018

Abstract

Bio-based industries require stable enzymes in a broad range of environmental conditions. Extremophiles have attracted more interests as the source of such enzymes, one of which is α-amylase. This study aimed to purify and characterize α-amylase produced by a thermo-halophilic bacterium PLS 75 isolated from underwater fumaroles. Ammonium sulfate precipitation results showed that the highest specific α-amylase activity (21.7 U/mg) obtained at 40-60% saturation level, with a purity of 7.7-fold of the crude extract with 16.2% yield. Further purification using DEAE Sepharose column chromatography increased the enzyme purity 11.1-fold of the crude extract with 7.1% yield. Specific activity after column chromatography purification was 31.3 U/mg. The pure enzyme had a low molecular weight of 14 kDa. The enzyme showed the highest activity at 80 °C and pH 5. The activity increased to 126% when in methanol, while decreased when in ethyl acetate and chloroform. The characteristics of α-amylase with low molecular weight, which was active in acidic condition, stable in polar and non-polar solvents, may be used for for specific industrial needs.

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Journal Info

Abbrev

biosaintifika

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological ...