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Biosaintifika: Journal of Biology & Biology Education
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,051 Documents
Biomass Production of Gynura procumbens Adventitious Roots in Different Type of Liquid Culture Manuhara, Yosephine Sri Wulan; Kusuma, Dannis Yuda; Sari, Rafika Lailiyatul Kurnia; Kristanti, Alfinda Novi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9670

Abstract

Gynura procumbens has a potency to produce raw material for pharmaceutical industry. Liquid culture systems have significant effects on multiplication rates of organ, so the propagation technology to increase biomass of this plant or organ using the liquid culture system was necessary. This research was conducted to compare biomass production of adventitious roots of G. procumbens in different liquid culture (shake flask, temporary immersion bioreactor and balloon-type bubble bioreactor). Adventitious roots culture was maintained in Murashige and Skoog liquid medium supplemented with IBA (indole butyric acid) 5 mg/L and different concentrations of sucrose (10, 30, 50 g/L). Result showed that the highest biomass production (fresh weight) was in shake flask that was 3.9-fold higher than initial explant, while in temporary immersion system was 5.12-fold higher than initial explant, and in balloon- type bubble bioreactor was 13.1-fold higher than initial explant. The highest enhancement of adventitious roots was occurred at sucrose supplementation of 50 g/L. Based on this research we found that the best type of liquid culture to increase biomass of G. procumbens adventitious roots was balloon-type bubble bioreactor. Scaling-up of adventitious root culture are necessary to fulfill the need of raw material for pharmaceutical industry and ballon-type bubble bioreactor was the most suitable method to scale up the performance.
The Effects of Dose Rhizoctonia Binucleat (BNR) and Phosphorus to Nitrate Reductase Activity (NRA) and Chlorophyll of Vanilla Seedling (Vanilla planifolia Andrews) Haryuni, Haryuni; Dewi, Tyas Soemarah Kurnia
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6328

Abstract

Vanilla (Vanilla planifolia Andrews) is one of the important exported commodities in Indonesia. Indonesia is one of top five major vanilla exporters in the world, that produce the high quality of Indonesian vanilla with high vanillin content (2.75%). The aims of this research were to determine the effects of dose binukleat Rhizoctonia (BNR) and phosphorus as well as the interaction of the nitrate reductase activity (NRA) and chlorophyll of the vanilla seedling (Vanilla planifolia Andrew). Method in this research used completely randomized factorial design, by involving two factors (dose of BNR inoculation and Phosphor). The first factor is without inoculation and inoculation BNR (M0, M1, M2, M3) wich consists of (0,5, 10, 15) g/polybag, the second factor is the dose of phosphorus fertilizer (P0, P1, P2, P3) which consists of (0, 3, 6, 9) g/polibag. The results showed that the inoculation dose of BNR and doses of phosphorus not significant and lower levels of NRA and chlorophyll while the interaction dose of BNR and phosphorus significantly and increase levels of NRA and chlorophyll of vanilla seedling. Nitrate Reductase Activity and chlorophyll has important role in metabolism process as a plant growth indicator.How to CiteHaryuni, H., & Dewi, T. S. K. (2016). The Effects of Dose Rhizoctonia Binucleat (BNR) and Phosphorus to Nitrate Reductase Activity (NRA) and Chlorophyll of Vanilla Seedling (Vanilla planifolia Andrews). Biosaintifika: Journal of Biology & Biology Education, 8(2), 141-147.
Pemberian Ekstrak Benalu Mangga terhadap Perubahan Histologis Hepar Tikus yang Diinduksi Kodein Maya Wijaya M, Sekar; -, Lisdiana; Setiati, Ning
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3103

Abstract

Benalu mangga mengandung senyawa kuersetin yang bertindak sebagai hepatoprotektor. Kodein merupakan jenis alkaloid dan banyak digunakan sebagai obat. Oversdosis kodein dapat menyebabkan kerusakan hepar. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak benalu mangga terhadap perubahan histologis hepar tikus yang diinduksi kodein. Sebanyak 20 ekor tikus wistar jantan dibagi menjadi 5 kelompok (K0, K, KB1, KB2, dan KB3). Kelompok K0 hanya diberi minum dan pakan standar, kelompok K diberi kodein 12mg/ekor selama 7 hari, selanjutnya hanya diberi pakan dan minum standar. Kelompok KB1 diberi kodein 12mg/ekor selama 7 hari dilanjutkan ekstrak benalu mangga 22mg/ekor sampai hari ke 21, kelompok KB2 diberi kodein 12mg/ekor selama 7 hari dilanjutkan ekstrak benalu mangga 44mg/ekor sampai hari ke 21, dan kelompok KB3 diberi kodein 12mg/ekor selama 7 hari dilanjutkan ekstrak benalu mangga 88mg/ekor sampai hari ke 21.Hari ke-27 semua tikus dibedah, diambil heparnya dan dibuat preparat histologi dengan pengecatan HE.Hasil penelitian kelompok K0 menunjukkan perubahan histologis hepar bermakna (p<0,05) antara K,KB1, KB2, dan KB3, sedangkan kelompok K tetapi tidak menunjukan perubahan bermakna (p>0,05) antara K,KB1, KB2, dan KB3. Pada kelompok K kerusakan struktur hepar meliputi inflamasi perportal dan degenerasi sedangkan pada kelompok KB1, KB2, dan KB3 kerusakan meliputi inflamasi periportal,degenerasi, dan nekrosis.Mangos mistletoes contain quercetin, a hepatoprotector. Codein is a type of alkaloids widely used as drug. Codein overdose can damage the liver. The objective of the study was know the effect of the administration of extract of mangos mistletoes on the changes of liver histology of codein-induced rats. As many as 20 male wistar rats were divided into 5 groups (K0, K, KB1, KB2, and KB3). K0 group were given only standard diet and water daily. K group were given codeine at 12mg/rats for 7 days, and afterwards they were given only standard diet and water daily. KB1 group were given codeine at 12mg/rats for 7 days and then were given extract of mangos mistletoes at 22 mg/rats until day 21, KB2 group were given codeine at 12mg/rats for 7 days and then were given extract of mangos mistletoes at 44 mg/rats until day 21, and KB3 group were given codeine at 12mg/rats for 7 days and were given extract of mangos mistletoes at 88 mg/rats until day 21. At day 27, all rats were killed, the livers were taken out for preparations with HE staining for histology examination. Research showed that K0 group was significantly different (p <0.05) to K, KB1, KB2, and KB3 groups, whereas K group was not significantly different to KB1, KB2 and KB3 groups. Rats in K group showed the changes in histological appearance such as periportal inflammation and degeneration, whereas rats in KB1, KB2, KB3 groups showed similar histological appearance but with necrosis.
Traditional Usages of Taro (Colocasia spp.) by Ethnic Communities in Borneo Oktavianingsih, Linda; Suharyanto, E.; Daryono, Budi Setiadi; Purnomo, Purnomo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9956

Abstract

Borneo has a a wealth of various flora, including the Aroids (Araceae). Taro is one of the Aroids that cultivated and used as food crop since a long time ago by the people in Borneo. This study aimed to determine the utilization of Taros traditionally by several ethnic communities in Borneo. The research used Survey Explorative Method with Direct Interview Technique in the field. Taro samples were taken from various habitats of Banjar, Dayak, Kutai, Malay, Bugis, Toraja and China ethnic. The results showed that Taro species widely used are : Colocasia esculenta var. esculenta and var. antiquorum, Colocasia affinis and wild of Colocasiaesculenta. C. esculenta var. esculenta and var. antiquorum is used as subsistence food crop and vegetables. Besides, it is used as medicine such as high blood pressure lowering and for consumption of diabetics. Parts of the plants consumed include leaves, petiole, corm and stolon. C. affinis is used as ornamental plants of the home garden, while wild C. esculenta is used as animal fodder. Taro in Borneo have a considerable variation of traditional cultivars and vernacular names. Fifty eight traditional cultivars from 5 different habitat to be used by some ethnics.
The Impact of Lampenflora on Cave-dwelling Arthropods in Gunungsewu Karst, Java, Indonesia Kurniawan, Isma Dwi; Rahmadi, Cahyo; Ardi, Tiara Esti; Nasrullah, Ridwan; Willyanto, Muhammad Iqbal; Setiabudi, Andy
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13991

Abstract

The development of wild caves into show caves is required an installation of electric lights along the cave passages for illumination and decoration purposes for tourist attraction. The presence of artificial lights can stimulate the growth of photosynthetic organisms such as lampenflora and alter the typical cave ecosystem. The study was aimed to detect the effect of lampenflora on cave-dwelling arthropods community. Four caves were sampled during the study, 2 caves are show caves with the existence of lampenflora and 2 others are wild caves without lampenflora. Arthropods sampling were conducted by hand collecting, pitfall trap, bait trap and berlese extractor. Lampenflora comprises of algae (Phycophyta), moss (Bryophyta) and fern (Pteridophyta) grow mostly around white light lamps. Richness, diversity, and evenness indices of Arthropods are higher in caves with the existence of lampenflora compared to caves without lampeflora. This study clearly shows that the presence of lampenflora can increase Arthropods diversity and suppress dominancy of common Arthropods species in caves, also increasing the relative abundance of predators. This condition will shift the ecosystem equilibrium and lead to cave ecosystem destruction. The results of this study should be a scientific consideration for show cave development and management. Lampenfloras have to be removed from all caves and preventive efforts should be taken to minimize their growth.
Optimasi Konsentrasi 2,4-D, Ba, dan Lama Penyinaran untuk Memacu Regenerasi Tunas dari Kalus Kedelai Kristanti, Intan; Habibah, Noor Aini; Herlina, Lina
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.3923

Abstract

Alternatif untuk mengatasi kualitas kedelai yang rendah yaitu perbaikan sifat genetik. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan Benzyl Adenine (BA) serta lama penyinaran dan interaksinya terhadap regenerasi tunas dari kalus kedelai, dan untuk menentukan interaksi faktor-faktor yang paling optimal dalam regenerasi tunas dari kalus kedelai. Konsentrasi 2,4-D dan BA masing-masing terdiri dari 4 taraf (0 ppm; 3 ppm; 6 ppm; 9 ppm) dan 2 taraf lama penyinaran (24 jam dan 0 jam). Analisis menggunakan ANAVA tiga arah dan uji lanjut Duncan. Parameter yang diamati adalah waktu muncul tunas, panjang tunas, jumlah tunas, dan persentase kalus membentuk tunas. Hasil menunjukkan bahwa konsentrasi BA dan lama penyinaran mempengaruhi regenerasi tunas dari kalus kedelai, sedangkan konsentrasi 2,4-D tidak berpengaruh signifikan terhadap regenerasi tunas. Konsentrasi BA yang paling optimal adalah 3 ppm dan lama penyinaran yang optimal adalah kondisi 0 jam. Interaksi konsentrasi BA, 2,4-D dan lama penyinaran berpengaruh terhadap regenerasi tunas terutama pada banyaknya jumlah tunas yang dihasilkan. Perlakuan BA 3 ppm + 2,4-D 6 ppm + 24 jam adalah perlakuan yang optimal dalam meregenerasi tunas dengan jumlah kalus yang banyak. Berdasarkan hasil penelitian disarankan untuk meregenerasi kalus menggunakan ZPT BA dan 2,4-D dan Lama penyinaran.Genetic trait improvement is a way to overcome the low quality soybean. The aim of this research is to determine the effect of 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Benzyl Adenine (BA) concentration and long irradiation and their interaction on bud regeneration from callus soybean, and to determine the interaction of the factors that most optimal in bud regeneration from callus soybean. 2,4-D and BA concentration each consisting of 4 levels (0 ppm; 3 ppm; 6 ppm; 9 ppm) and 2 levels long irradiation (24 hours and 0 hour). Analysis using a three -way ANAVA and Duncan test further. Parameters measured were the time appears bud, bud length, number of buds, and the percentage of callus forming buds. The result showed that BA concentration and long irradiation affects the regeneration of shoots from callus soybean, whereas the concentration of 2,4-D had no significant effect on bud regeneration. The most optimal concentration of BA is 3 ppm and optimal long irradiation is the condition 0 hours. BA concentration, 2,4-D concentration and long irradiation interaction effect on the regeneration of buds mainly on the number of buds produced. Treatment BA 3 ppm + 2,4-D 6 ppm + 24 hours is the optimal treatment in regenerating buds the number of callus that many. Based on the research results suggested to regenerate callus using ZPT BA and 2,4-D and long irradiation.
TINGKAT KEGANASAN KANKER SERVIKS PASIEN PRA-RADIASI MELALUI PEMERIKSAAN AgNORs, MIB-1 DAN Cas- 3 Kurnia, Iin; Bintari, Siti Harnina; Khaisuntaha, Mafaza
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2269

Abstract

Kanker serviks sering ditemukan di negara berkembang. Pengobatan kanker melalui radioterapi untuk mengetahui tingkat proliferasi dan mengurangi tingkat keganasan. Biomarker proliferasi dan apoptosis berupa AgNORs, MIB-1, dan Caspase 3. Namun belum dijelaskan mengenai korelasi ketiga biomarker dalam kaitannya dengan proliferasi dan apoptosis pada sel kanker serviks. Tujuan penelitian untuk mengetahui korelasi antara AgNORs, MIB-1, dan apoptosis pada kanker serviks. Penelitian observasional laboratoris menggunakan metode pewarnaan dengan menekankan kontras warna antara sitoplasma dan inti sel. Objek berupa sediaan mikroskopis dari 30 biopsi pasien kanker serviks. Pengambilan data dengan metode crocker dan blind manner. Analisis data menggunakan uji korelasi, dari ju mlah 21 pasien yang diamati menunjukkan. AgNORs dan MIB-1 memiliki angka relatif tinggi. Angka yang diperoleh ini berbanding terbalik dengan apoptosis yang relatif rendah. Korelasi antara AgNORs dengan MIB-1 menunjukkan r= 0,33 dan p= 0,15. AgNORs dengan apoptosis memiliki korelasi negatif yakni, r=-0,08 dan p= 0,73. MIB-1 dengan apoptosis memiliki korelasi negatif pula r= -0,18 dan p= 0,43. Kesimpulannya korelasi AgNORs dengan apoptosis memiliki kecenderungan lebih baik dari pada MIB-1 dengan apoptosis.Cervical cancer is often found in the developing countries. The treatment of cancer through radiotherapy was performed to determine the proliferation level and to reduce the malignancy level of cancer. The proliferation and apoptotic biomarkers were AgNORs, MIB-1, and Cas- 3. However, the correlation between the three biomarkers in relation to the proliferation and apoptosis in cervical cancer cells was not clear. The purpose of the study was to determine the correlation between AgNORs, MIB-1 and apoptosis in cervical cancer. This study was an observational research laboratory using a staining method to emphasize the color contrast between the cytoplasm and the nucleus of the cells. The microscopic preparations of the 30 patients with cervical cancer biopsies had been used as the study objects. Data was collected using the Crocker and Blind method and was then analyzed using correlation test. Data from 21 patients with AgNORs and MIB-1 showed a relatively high value. The figure obtained was inverse proportionally to the relatively low apoptotic value. The correlation between AgNORs and MIB-1 showed r = 0.33 and p = 0.15. There was negative correlation between AgNORs and apoptosis at r = -0.08 and p = 0.73. Meanwhile, between MIB-1 and apoptosis has also a negative correlation at r = -0.18 and p = 0.43. It was concluded that the correlation between AgNORs and apoptosis tended to be better than the MIB-1 and apoptosis.
Contribution of Plantation Forest on Wild Bees (Hymenoptera: Apoidea) Pollinators Conservation in Mount Slamet, Central Java, Indonesia Widhiono, Imam; Sudiana, Eming; Yani, Edy
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10652

Abstract

Wild bee pollinators (Hymenoptera : Apiade) diversity and abundance were studied in three types of plantation forest on Mt. Slamet (Central Java Province, Indonesia). The aims of the research was to know the diversity and abundance of wild bee pollinators and to determine the possibility of plantation forest contribution on wild bees conservation. Sampling has been done at three stands: a pine forest (PF, with Pinus merkusii), an Agathis forest (AF, with Agathis damara) and a community forest (CF, with Albizia falctaria). Each habitat was divided into 5 line transect (100 x 5 m) and sweep nets were used to collect the wild bee samples. Sampling was done eah month from April to August 2015. The diversity of wild bees was high (12 species in 9 genera; members of the Apidae (7 species were dominant). The most abundant species across the forests were Apis cerana (343 individuals; 25.5% of total), Trigona laeviceps (195 individuals; 14.5%), and Megachille relativa (165 individuals; 12.3%). Measurements of species diversity (H), species evenness (E), habitat similarity (Ss) and species richness indicated that the wild bee species diversity in the region was relatively high (H = 1.275) to (H = 1.730);(E= 0.870) to (E = 0.93). The result showed that the diversity of wild bees in three different plantation forest habitats on Mt. Slamet were similar and can be concluded that plantation forest types were important for pollinator conservation, and an appropriate future preservation strategy should include of the areas of all plantation forest types.
The Potency of Black Soldier Larvae (Hermetia illucens L.) as a Source of Protein for Livestock Feed Supriyatna, Ateng; Kurrahman, Opik Taufik; Cahyanto, Tri; Yuliawati, Astri; Kulsum, Yuni
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14422

Abstract

Black Soldier fly larvae is an insect capable to convert organic waste into body biomass. The purpose of this research was to determine the productivity, mass balance of the consumption process, and content of protein and fat of prepupa which has a potency as a livestock feed. This research used 6-day-old larvae, they were fed with cassava skin that has been smoothed with a variety of feeding doses: 12.5; 25; 50; 100; and 200 (mg / larvae / day) with ratio of feed and water 2: 1. The results showed that the feeding of 200 mg / larva / day produced the best larval growth with biomass productivity of 1.54 mg / day, residue yielded of 67.1 mg / day. The organic waste was convertion into larvae biomass. In this study, approximately consumption process scale is feed successfully converted to biomass 2.77%, metabolism 6.98%, and 90.24% residue. Protein content is 25.7%. It can be concluded that the larvae are able to convert organic waste into high protein biomass and potentially being used as a livestock feed. This research acts a model to predict mass balance of substrates for feed. The benefit of this research is the science approach used in this study that can be used as a reference by researchers and the community to determine the biomass production of an organism.
Bird Communities In Seblat Nature Recreation Park (SNRP) North Bengkulu, Bengkulu Susanto, Eki; Mulyani, Yeni Aryati; Suryobroto, Bambang
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4948

Abstract

Bird communities are the composition of several bird species that live together in the same place, time and interact with other birds. The diversity of birds in an area can be used as an indicator of stabilizing an area. Changes of vegetation structures due to logging practices can affect the availability of resources for bird communities. The objective of the research was to examine diversity, richness, bird species abundance and bird community similarity at HS1 (logged forest 1974), HS2 (logged forest 1989/1990) and HS3 (fully logged forest 1989/1990) in Seblat Nature Recreation Park (SNRP). The research was conducted in July September 2013. Data collection was conducted by point count method (total 36 points) and mist net method (total 4752 nets hours). The Research showed 85 birds species from 33 families were recorded. HS2 was highest value of diversity and richness (H = 3.63, DMG = 10.07). The highest relative abundance species in HS1 was Emerald Dove (Chalcophaps indica), while HS2 and HS3 were Slender-billed Crow (Corvus enca). The bird community similarity was highest in HS2 and HS3 (ISj = 0.58).How to CiteSusanto, E., Mulyani, Y. A., & Suryobroto, B. (2016). Bird Communities In Seblat Nature Recreation Park (SNRP) North Bengkulu, Bengkulu. Biosaintifika: Journal of Biology & Biology Education, 8(1), 25-32.

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