Current Biochemistry Journal
Vol. 8 No. 2 (2021)

Analysis of Metabolite Compound Profiles of Miana Leaves Endophytic Bacteria (Coleus scutellariodes) using GC-MS

Dwi Endah Kusumawati (Program Studi Farmasi, Fakultas Kedokteran, Universitas Islam Sultan Agung Semarang)
Ukhradiya Magharaniq Safira Purwanto (Department of Biochemistry, IPB University)
Maria Bintang (Department of Biochemistry, IPB University)
Fachriyan Hasmi Pasaribu (Unknown)



Article Info

Publish Date
26 Feb 2022

Abstract

Exploration of bioactive compounds from endophytic bacteria as novel source of antibacterial need to be explored, as an alternative to substitute synthetic antibiotics. Based on previous studies, DM6 was a potential isolate that showed antibacterial activity. The isolate has been identified as Brevibacillus sp. based on 16S rRNA analysis. This study aimed to identify antibacterial compounds produced by DM6 isolate using GC-MS. The GC-MS results showed that the chloroform extract of DM6 isolate contained : 1-Octadecene (CAS).alpha.-Octadecene, Hexanedioic acid, dioctyl ester (CAS) Dioctyl adipate, Phenol, 3,5-bis(1,1-dimethylethyl)- (CAS) 3,5-Di-tert-butylphenol, and 1,4-diaza-2,5-dioxobicyclo[4.3.0]nonane. Adipic acid/hexanedioic acid and phenols are also known to contained in the leaves of Coleus scutellariodes. This indicated that endophytic bacteria were able to synthesize antibacterial compounds which similar with the host plant.

Copyrights © 2022






Journal Info

Abbrev

cbj

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Education

Description

Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, ...