Dental Journal (Majalah Kedokteran Gigi)
Vol. 55 No. 1 (2022): March 2022

Tumor necrosis factor-α and osterix expression after the transplantation of a hydroxyapatite scaffold from crab shell (Portunus pelagicus) in the post-extraction socket of Cavia cobaya

Irvan Salim (Faculty of Dental Medicine, Universitas Airlangga, Surabaya)
Michael Josef Kridanto Kamadjaja (Faculty of Dental Medicine, Universitas Airlangga, Surabaya)
Agus Dahlan (Faculty of Dental Medicine, Universitas Airlangga, Surabaya)



Article Info

Publish Date
01 Mar 2022

Abstract

Background: Socket preservation using bone graft is one way to minimise resorption and maximise the bone formation process. Tumor necrosis factor-α (TNF-α) is an inflammatory cytokine that affects bone regenerating osteoblast activity, while osterix (Osx) is an osteoblast-specific transcription factor that activates gene receptors during pre-osteoblast differentiation. The hydroxyapatite (HA) scaffold from crab shells (Portunus pelagicus) has osteoconduction properties. Purpose: To analyse the decrease of TNF-α expression and the increase of Osx expression and the correlation between these two in the post-extraction socket after the transplantation of a crab shell HA scaffold. Methods: The lower left incisors of Cavia cobaya (n = 24) were extracted and divided into four groups: the first and second groups were control groups on Day 7 and Day 14 (K7 and K14), the third and fourth groups were treatment groups (P7 and P14). The statistical analysis used was a multivariate analysis of variance (MANOVA) with a significance level of 0.05. Results: A MANOVA test showed that the use of crab shell HA scaffolds led to a significant difference (p < 0.05) in TNF-α expression (p = 0.01) and Osx expression (p = 0.01). A Pearson correlation test result showed a strong inverse correlation between TNF-α and Osx expressions (p = 0.00 and r = -0.78). Conclusion: The transplantation of HA scaffolds from crab shells can decrease TNF-α expression but increase Osx expression in the post-extraction socket of C. cobayas. Furthermore, an inverse correlation was found between TNF-α and Osx.

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Journal Info

Abbrev

MKG

Publisher

Subject

Dentistry

Description

The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly ...