Jurnal Respirologi Indonesia
Vol 42, No 2 (2022)

Immune Checkpoint Marker of Programmed Death Ligand-1 (PD-L1) Expression in Surgical Lung Cancer Specimens

Elisna Syahruddin (Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Indonesia)
Jamal Zaini (Universitas Indonesia)
Lisnawati Lisnawati (Universitas Indonesia)
Yayi DB Susanto (Universitas Indonesia)
Sarah Fitriani (Human Cancer Research Center, Indonesia Medical Education and Research Institute)
Shanty R. Kusumawardani (Human Cancer Research Center, Indonesia Medical Education and Research Institute)
Romi Baginta (Universitas Indonesia)



Article Info

Publish Date
18 Apr 2022

Abstract

Background: Currently immune checkpoint pathways of PD1-PD-L1 is being used to treat lung cancer and PD-L1 served as predictive biomarker. Investigations of PD-L1 expression as a target immunotherapy in lung cancer specimens in Indonesia is needed. This study aimed to evaluate PD-L1 expression in resected lung cancer specimens using immunohistochemistry techniques. Methods: Thirty surgically resected sample from lung cancer patients were obtained. The whole specimens were stained by using immunohistochemistry techniques automatically in BOND-MAX Autostainner (Leica, Germany). PD-L1 polyclonal antibody (Genetex) at 1:1500 dilution was applied to immunohistochemistry staining procedure. Clinicopathological characteristic were acquired from the hospital registry database.Result: A total of 30 surgical specimens were assessed from lung cancer patients. Twenty-four (80%) of them were Adenocarcinoma (AC), 1 (3, 33%) Adenosquamous carcinoma, 2 (6, 67%) were Squamous Cell Carcinoma (SSC), 1 (3, 33%) were Large Cell Carcinoma, 1 (3, 33%) were Neuroendocrine Carcinoma, 1 (3, 33%) were Adenoid Cyctic Carcinoma. The expression of PDL-1 positive reactivity was 16 of 30 (53.3%) specimens and we categorized into strong positivity (>50%), medium (= 50%) and low positivity (<50) from 21 specimens, 0 specimens and 7 specimens respectively.Conclusion: PD-L1 expression could be detected in lung cancer specimen using polyclonal antibody.

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Journal Info

Abbrev

jri

Publisher

Subject

Health Professions Medicine & Pharmacology

Description

Jurnal Respirologi Indonesia (JRI) is an online and printed scientific publication of the Indonesian Society of Respirology (ISR). The journal is published thrice-monthly within a year (January, April, July and October). The journal is focused to present original article, article review, and case ...