Pharmacy Reports
Vol. 1 No. 1 (2021): Pharmacy Reports

The detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP) in developing country

Muhammad Rizky Wibowo (Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia)
Erna Harfiani (Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia)
Sarmoko Sarmoko (Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman, Indonesia)
Yudhi Nugraha (Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia)



Article Info

Publish Date
31 Aug 2021

Abstract

The severe acute respiratory syndrome coronavirus (SARS-CoV-2) has infected the human system resulting in Covid-19, and has spread rapidly worldwide. Therefore, a fast, simple, cost-effective, and accurate detecting tool is required. The standard diagnostic tool of the World Health Organization is the reverse transcription-polymerase chain reaction (RT-PCR). This method detects the presence of viral genetic material in the human body with accurate results. However, it has several limitations in terms of equipment, personnel, duration, and cost. Therefore, a fast, simple, and sensitive alternative detection, is required, one of which is the reverse transcription loop-mediated isothermal amplification (RT-LAMP) that functions under isothermal conditions. This method is battery-driven, hence, easy to move closer to the patient. Conclusively, the RT-LAMP test for SARS CoV-2 diagnosis produces comparable sensitivity to a standard RT-PCR and is more suitable for resource-poor settings, such as rural areas of developing countries.

Copyrights © 2021






Journal Info

Abbrev

pharmrep

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

Description

Pharmacy Reports is an open-access journal publishing peer-reviewed research in the pharmacy field, covering topics in pharmaceutics, biomedicine, pharmaceutical chemistry, bioinformatics, natural product, pharmacology and toxicology, and clinical pharmacy. Pharmacy Reports invites you to submit ...