Microbiology Indonesia
Vol. 2 No. 1 (2008): April 2008

Isolation and Identification of Ice-Nucleating-Active Bacteria from Indonesian Edible Leafy Plant Poh-Pohan (Pilea glaberina)

DIANA ELIZABETH WATURANGI (Universitas Katolik Indonesia Atma Jaya)
VICKY MEICY (Universitas Katolik Indonesia Atma Jaya)
ANTONIUS SUWANTO (Institut Pertanian Bogor)

Article Info

Publish Date
13 Mar 2010

Abstract

Two ice-nucleating-active (INA) bacteria (isolates C and 6) were isolated from poh-pohan (Pilea glaberina), an Indonesian edible leafy plant (lalaban). The maximum nucleation temperature of aqueous suspensions of the two isolates is -5 °C. They were classified as a type II ice nucleator. Microscopic and morphological determination showed that these isolates had yellow pigmentation, rod shape, and were Gram negative. Biochemical analysis indicated that the isolates were exhibited catalase activity, but negative in oxidase and indole assays. DNA sequencing of 16SrRNA gene of isolate A3 showed a 94% similarity to Pseudomonas sp. while isolate A4 showed a 97% similarity to Xanthomonas campestris. To our knowledge, this is the first report of INA bacteria isolated from a tropical edible leafy plant.

Copyrights © 2008




Citation Download
RIS
EndNote, Reference Manager, ProCite
BibTex
Latex, Jabref
Full PDF
39.169 KB
Original Source
Download Original
Google Scholar
Check in Google Scholar
Journal Info
Microbiology Indonesia
Website

Abbrev

mionline

Publisher

Perhimpunan Mikrobiologi Indonesia

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, ...