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Isolation, Characterization, and Genetic Diversity of Ice Nucleation Active Bacteria on Various Plants DIANA ELIZABETH WATURANGI; AMELIA TJHEN
HAYATI Journal of Biosciences Vol. 16 No. 2 (2009): June 2009
Publisher : Bogor Agricultural University, Indonesia

Ice nucleation active (INA) bacteria is a group of bacteria with the ability to catalyze the ice formation at temperature above -10 oC and causing frost injury in plants. Since, most of the literature on INA bacteria were from subtropical area, studies of INA bacteria from tropical area are needed. We sampled eight fruits and 36 leaves of 21 plant species, and then identified through biochemical and genetic analysis. INA bacteria were characterized for INA protein classification, pH stability, and optimization of heat endurance. We discovered 15 INA bacteria from seven plants species. Most of bacteria are oxidase and H2S negative, catalase and citrate positive, gram negative, and cocoid formed. These INA bacteria were classified in to three classes based on their freezing temperature. Most of the isolates were active in heat and pH stability assay. Some isolates were analysed for 16S rRNA gene. We observed that isolates from Morinda citrifolia shared 97% similiarity with Pseudomonas sp. Isolate from Piper betle shared 93% similarity with P. pseudoalcaligenes. Isolate from Carica papaya shared 94% similarity with Pseudomonas sp. While isolate from Fragaria vesca shared 90% similarity with Sphingomonas sp. Key words: INA bacteria, isolation, characterization, genetic diversity, plants

Antibiotic Resistance and Integron of Vibrio cholerae Detection from School Street Foods in Jakarta NADIA DEASHINTA; DIANA ELIZABETH WATURANGI; YOGIARA YOGIARA
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

Street foods represent foods and beverages prepared by vendors in streets or other public places, i.e. schools. Food safety issues perceive street foods as a potential major public risk. Street foods contaminated with toxigenic Vibrio cholerae may lead to serious poisoning to school-age children. In this study, 17 isolates of V. cholerae were obtained from nine (45%) of total 20 street foods samples collected in Jakarta. Five (29%) were confirmed to be V. cholerae O1, serotype Ogawa using biochemical tests and serological identification. Of the 17 V. cholerae isolates 47% proved to be resistant to ampicillin, 35% to trimethoprim, 17.6% to tetracycline, and 17.6% to streptomycin. A class 1 integrons bearing streptomycin/spectinomycin resistant gene cassette of aadA1c were discovered on isolate Vc25n. This may leads to horizontal transfer of the antibiotic resistant genes to other bacteria. Key words: foods, Vibrio cholerae, antibiotic resistance, integron

The human feet and mouth are known as sources of methylated sulfides, which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria, which may result in odor reduction in human feet and mouth. In this study, we collected a total of 21 isolates from human feet, and 37 isolates from human mouth. These isolates were identified with biochemical test such as oxidase and catalase test and Gram staining assay. The presence of mxaF gene of methanol dehydrogenas DIANA ELIZABETH WATURANGI; CATHERINE DELANY NICHOLAS; CINDY OKTAVIA SUSANTO; MAGGY THENAWIJAYA SUHARTONO
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.11

The human feet and mouth are known as sources of methylated sulfides, which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria, which may result in odor reduction in human feet and mouth. In this study, we collected a total of 21 isolates from human feet, and 37 isolates from human mouth. These isolates were identified with biochemical test such as oxidase and catalase test and Gram staining assay. The presence of mxaF gene of methanol dehydrogenase was detected by PCR using specific primers. However, the result showed that most of the isolates did not possess mxaF gene. Hence, the methanol dehydrogenase (MDH) activity was also determined. From the total 21 isolates obtained from the feet, only 15 of them showed MDH activity whereas 23 isolates from the total 37 isolates obtained from teeth and tongue region also showed MDH activity. Isolate K25-3 (74.444 U/ml), K33-6 (79.815 U/ml), and K43-5 (69.259 U/ml) from human feet and M41L3 (135.926 U/ml), M27G2 (85.556 U/ml), and M51G1 (103.333 U/ml) from human mouth showed the highest total enzyme activity. Isolates with the highest total activity could be used for further studies such as purification of the enzyme and isolates characterization.

Methylotrophs inhabit the human mouth. In this study, methylotrophic bacteria were isolated from the human mouth microflora of 63 subjects, especially from the tongue, gingival, and subgingival area using minimal agar supplemented with 1% methanol. The obtained isolates were subjected to biochemical assays, continued with antibiotics susceptibility testing using ampicillin (10 mg), tetracycline (20 mg), kanamycin (30 mg), trimethoprim (5 mg), and streptomycin (10 mg). Genetic diversity was analy DIANA ELIZABETH WATURANGI; IVANA FRANCISCA IVANA FRANCISCA; CINDY OKTAVIA SUSANTO
HAYATI Journal of Biosciences Vol. 18 No. 2 (2011): June 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.2.77

Methylotrophs inhabit the human mouth. In this study, methylotrophic bacteria were isolated from the human mouth microflora of 63 subjects, especially from the tongue, gingival, and subgingival area using minimal agar supplemented with 1% methanol. The obtained isolates were subjected to biochemical assays, continued with antibiotics susceptibility testing using ampicillin (10 mg), tetracycline (20 mg), kanamycin (30 mg), trimethoprim (5 mg), and streptomycin (10 mg). Genetic diversity was analyzed using ARDRA method. Isolates varying in morphology characteristics were amplified for 16S rRNA gene and continued with DNA sequencing. As many as 21 methylotrophic bacterial isolates were purified and divided into seven groups with different phenotypic profiles. A majority of the isolates were resistant to trimethoprim but sensitive to kanamycin, streptomycin, and tetracycline. Resistance to ampicillin was variable in each isolate. ARDRA showed nine different digestion profiles. DNA sequencing analysis of the 16S rRNA gene showed that six isolates with different phenotypic and digestion profiles were closely related to Methylobacterium radiotoleran (94%), Microbacterium esteraromaticum (99%), Pseudomonas sp. (100%), and three of them were exhibited 99, 99, and 98% sequence similarity with Gordonia sp., respectively. The results of this study revealed diversity among methylotrophic bacteria particularly in human mouth.

Certain bacteria that induce biological ice nucleation are suspected to play an important role in condensation and ice nuclei formation in clouds. Those bacteria can produce biological ice nucleator1, which is a protein and usually found on leaf surface and air. Most studies on INA bacteria were conducted in subtropical areas. In this study, INA bacteria were isolated from rain-water and air between March to May 2008 from Jakarta, Bogor, Bekasi, Tangerang, and Depok. The percentage of INA bacter . STEPHANIE; DIANA ELIZABETH WATURANGI
HAYATI Journal of Biosciences Vol. 18 No. 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.3.108

Certain bacteria that induce biological ice nucleation are suspected to play an important role in condensation and ice nuclei formation in clouds. Those bacteria can produce biological ice nucleator1, which is a protein and usually found on leaf surface and air. Most studies on INA bacteria were conducted in subtropical areas. In this study, INA bacteria were isolated from rain-water and air between March to May 2008 from Jakarta, Bogor, Bekasi, Tangerang, and Depok. The percentage of INA bacteria from rainfall for those area are 19.4, 18.7, 5.3, 2.2, and 6.4% respectively, while percentage for air are 9.5, 6.5, 0, 2.7, and 1.8%. The highest incidence of INA bacteria were from rain-water and air found in sample from Jakarta and then followed by the samples from Bogor. It was shown that the percentage of INA bacteria from rain-water was higher than air for all of the samples from different areas. The isolate from Jakarta (isolate A32) which had the highest activity for ice nucleation, with the temperature classification at -2.7 oC, revealed 100% similarity with Pantoea sp. The presence of INA bacteria in rain-water and air might play an important role in nucleation process which is required for rainfall induction.

Isolation and Identification of Ice-Nucleating-Active Bacteria from Indonesian Edible Leafy Plant Poh-Pohan (Pilea glaberina) DIANA ELIZABETH WATURANGI; VICKY MEICY; ANTONIUS SUWANTO
Microbiology Indonesia Vol. 2 No. 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

Two ice-nucleating-active (INA) bacteria (isolates C and 6) were isolated from poh-pohan (Pilea glaberina), an Indonesian edible leafy plant (lalaban). The maximum nucleation temperature of aqueous suspensions of the two isolates is -5 °C. They were classified as a type II ice nucleator. Microscopic and morphological determination showed that these isolates had yellow pigmentation, rod shape, and were Gram negative. Biochemical analysis indicated that the isolates were exhibited catalase activity, but negative in oxidase and indole assays. DNA sequencing of 16SrRNA gene of isolate A3 showed a 94% similarity to Pseudomonas sp. while isolate A4 showed a 97% similarity to Xanthomonas campestris. To our knowledge, this is the first report of INA bacteria isolated from a tropical edible leafy plant.

Identification of Class 1 Integron of Escherichia coli from Street Foods in Jakarta . FRISCA; BIBIANA WIDYATI LAY; DIANA ELIZABETH WATURANGI
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

A total of 43 Escherichia coli isolates were identified from school street foods located in Northern and Southern Jakarta. The isolates were examined for antibiotic resistance using five antibiotics discs (ampicillin, kanamycin, streptomycin, trimethoprim, tetracycline) and screened for the class 1 integron with specific conserved region primer using PCR amplification. The antibiotic diffusion test revealed three isolates (7%) with resistance to multiple antibiotics. PCR detection of integron regions showed one isolate possessed a class 1 integron bearing one gene cassette with ~700bp amplicon size. DNA sequencing showed that the gene cassette was resistant to trimethoprim determinant type V (dhfrV). The integron bearing E. coli strain could become a threat for the widespread distribution of an antibiotic resistance gene especially for pathogenic bacteria.

Analysis of Pink Pigmented Facultative Methylotroph Bacteria from Human Environments DIANA ELIZABETH WATURANGI; ANDREAS KUSUMA
Microbiology Indonesia Vol. 2 No. 3 (2008): December 2008
Publisher : Indonesian Society for microbiology

The formation of pink biofilm in wet places are usually correlated with chlorine-resistant pink pigmented facultative methylotrophs (PPFM). In this study we investigated the presence of PPFM bacteria through bacterial isolation and detection of mxaF gene from wet places of human-made environments. A total of eighteen PPFM bacterial isolates were recovered from the formation of biofilm bacterial of four test places such as washstands, bathrooms, and potable water supplies. Confirmation of the isolates through biochemical analysis were done using catalase, oxidase and urease tests. Chlorine-resistance-activity was assayed for all of the isolates. Antibiotic resistance were examined for ampicillin (25 μg), tetracycline (30 μg), kanamycin (30 μg), trimethoprim (1.25 μg), and streptomycin (10 μg) using the agar diffusion method. Genomic DNA was subjected to PCR analysis with primers corresponding to the 5’- and 3’- end conserved segments of the mxaF gene. PCR amplification followed by DNA sequencing of 16S rRNA gene were done for some isolates. We recovered 18 isolates of PPFM bacteria. Biochemical analysis indicated that the isolates were positive for catalase, oxidase, and urease activities. Chlorine-resistance-analysis showed the majority of the isolates were resistant to chlorine. Antibiotic resistance assays showed all of the isolates exhibited resistance to trimethoprim but were sensitive to streptomycin, kanamycin, and tetracycline but were variably resistant to ampicilin. PCR detection using specific primers for the mxaF gene gave a positive result for all of the isolates. DNA sequencing of the 16S rRNA gene of two isolates showed that isolate WD10 had a 98% similarity with the mxaF gene from Methylobacterium lusitanum strain MP2 and isolate WK2 had a 98% similarity to the mxaF gene from Afipia felis strain RD1. The formation of pink biofilm of four wet areas in this study were correlated with the presence of chlorine-resistant PPFM bacteria and we confirmed with the presence of the mxaF gene in all of the isolates. This finding needs to be widely publicized since some PPFM bacteria were known as opportunistic pathogens.

EDUKASI PADA MASYARAKAT MENGENAI PERAN MIKROORGANISME DALAM PERUBAHAN IKLIM Daru Seto Bagus Anugrah; Dionysius Subali; Diana Elizabeth Waturangi; Rory Hutagalung; Jessica Igna Amanda
Jurnal Bakti Masyarakat Indonesia Vol 4, No 2 (2021): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jbmi.v4i2.12624

Climate change is a hot topic and attracts great attention because it causes a series of side effects for the environment and living organisms in the world. Microorganisms are involved in many processes in nature that can affect climate change, including the complex cycle of phosphorus, sulfur, nitrogen, oxygen, and carbon. Microorganisms also play a role in producing and reducing greenhouse gases such as carbon dioxide and methane. In addition, microorganisms can respond positively or negatively to temperature. Understanding the activity of microorganisms is very important to know their functions and uses in the development of technologies that can combat climate change itself. Currently, the general public does not yet know the functions and uses of microorganisms to combat climate change. In collaboration with the American Society for Microbiology and Nutrienvisus Technologies, the Faculty of Biology, Atma Jaya Catholic University of Indonesia, conducts community service activities through online seminars on the role of microorganisms in climate change. Seminars are held online using the Zoom conference app. The participants in this activity were 210 people, including twenty-three high school students, seventy-six students, one housewife, seventy-two lecturers, four private employees, fourteen civil servants, and ten researchers. The forms of activities carried out were the presentation of the material, question and answer sessions, and the provision of questionnaires to the participants before and after the presentation of the material. The materials given are Soil Microbiomes and Climate Changes and Bacterial Ice Nucleation, and Air Microbiology. Speakers provide material based on the literature and the results of research that has been done. Questionnaires were administered online using the Mentimeter and Kahoot apps. The questionnaire results showed an increase in participants' understanding of the role of microorganisms in climate changeABSTRAK:Perubahan iklim menjadi topik yang menarik perhatian yang besar karena menyebabkan serangkaian efek samping bagi lingkungan maupun organisme hidup di dunia. Mikroorganisme terlibat pada banyak proses di alam yang dapat mempengaruhi perubahan iklim antara lain siklus kompleks fosfor, sulfur, nitrogen, oksigen dan karbon. Mikrooganisme juga berperan dalam menghasilkan dan mengurangi gas rumah kaca seperti karbon dioksida dan metana. Selain itu, mikroorganisme dapat memberikan respon positif maupun negatif terhadap suhu. Memahami aktivitas mikrooganisme sangat penting untuk mengetahui fungsi dan kegunaan dalam pengembangan teknologi yang dapat memerangi perubahan iklim itu sendiri. Saat ini, masyarakat umum belum mengetahui fungsi dan kegunaan mikroorganisme sebagai teknologi dalam memerangi perubahan iklim. Bekerja sama dengan American Society for Microbiology dan Nutrienvisus Technologies, Fakultas Teknobiologi Universitas Katolik Indonesia Atma Jaya melakukan kegiatan pengabdian masyarakat (PKM) melalui seminar secara daring mengenai peran mikroorganisme dengan perubahan iklim. Seminar diadakan secara daring menggunakan aplikasi konferensi Zoom. Peserta yang mengikuti kegiatan ini sejumlah 210 orang meliputi 23 orang siswa SMA, 76 orang mahasiswa, 1 orang ibu rumah tangga, 72 orang dosen, 4 orang karyawan swasta, 14 orang PNS, dan 10 orang peneliti. Bentuk kegiatan yang dilakukan adalah pemaparan materi, sesi tanya jawab, dan pemberian kuesioner kepada para peserta sebelum dan sesudah pemaparan materi. Materi yang diberikan adalah Soil Microbiomes and Climate Changes dan Bacterial Ice Nucleation and Air Microbiology. Pembicara memberikan materi berdasarkan literatur dan hasil penelitian yang telah dilakukannya. Kuesioner diberikan secara daring menggunakan aplikasi Mentimeter dan Kahoot. Hasil kuesioner menunjukkan terjadi peningkatan pemahaman peserta terhadap peran mikroorganisme terhadap perubahan iklim

Screening of Antibiofilm Activity from Marine Bacteria against Pathogenic Bacteria ALIANDA BUDHIRIANi ROSSATI CAMESI; AGUSTINA LUKITO; DIANA ELIZABETH WATURANGI; HWANG JAE KWAN
Microbiology Indonesia Vol. 10 No. 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

Bacterial biofilms produced by pathogenic bacteria have become a serious issue in several chronic diseases such as atherosclerosis, cystic fibrosis, endocarditis, inner ear infections, and kidney stones. Thus, inhibition and destruction of bacterial biofilm from pathogenic bacteria is needed. The purpose of this study is to analyze biofilm inhibition and destruction activities of marine bacteria associated with hard and soft corals isolated from several oceanic regions in Indonesia. Fifteen marine isolates collected from several regions in Indonesia such as Bali Province, South East Sulawesi Province, East Java Province, Lampung Province, and Banten Province were tested using static biofilm assay against several pathogenic bacteria. Biofilm of the pathogenic bacteria tested were stained using 0.4% crystal violet. Several isolates were sequenced using 16S rRNA PCR method. Most of marine isolates presented higher inhibition and or destruction activity at 10% crude concentration. Few isolates were further identified using 16S rRNA and proven to have antibiofilm activity against several pathogenic bacteria. Marine bacteria have broad applications in medical and pharmaceutical industries and the oceanic regions of Indonesia are promising sources for the discovery of novel bacteria with antibiofilm activity.

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