ABSTRACTCogongrass roots are known to have some metabolite content, which one is polyphenols. Cogongrass roots are often used as medicine for several diseases such as bloody urine, gonorrhea, vomiting blood, nosebleeds, acute hepatitis, acute renal inflammation, also hypertension. Usually, cogongrass roots were boiled before being used as a medicine. This study aimed to determine the difference in total phenol content in boiled water of cogongrass roots that were boiled at various times. This study was a pre-experiment with the one-shot case study design. The cogongrass roots were boiled for 5, 15, and 30 minutes. This study showed that the average total phenolic content that boiled in 5 minutes is 169,3 mg/L GAE, 15 minutes is 173,1 mg/L GAE, and 30 minutes is 177,2 mg/L GAE. The One Way ANOVA test showed a difference in total phenol content in boiled water of cogongrass roots at various boiling times. The Least Significant Difference (LSD) test showed a significant difference in total phenol content at each boiling time. This study concludes that there is a difference in total phenol content in boiled water of cogongrass roots based on boiling time.ABSTRAKAkar alang-alang diketahui memiliki beberapa kandungan metabolit, salah satunya adalah polifenol. Akar alang-alang sering digunakan sebagai obat beberapa penyakit seperti kencing berdarah, kencing nanah, muntah darah, mimisan, hepatitis akut, radang ginjal akut, juga hipertensi. Biasanya akar alang-alang direbus sebelum digunakan sebagai obat. Penelitian ini bertujuan untuk mengetahui perbedaan kadar fenol total pada air rebusan akar alang-alang yang direbus pada berbagai waktu. Penelitian ini merupakan penelitian pra-eksperimen dengan desain studi kasus one-shot. Akar alang-alang direbus selama 5, 15, dan 30 menit. Hasil penelitian menunjukkan bahwa rata-rata kadar fenolik total yang direbus dalam 5 menit adalah 169,3 mg/L GAE, 15 menit adalah 173,1 mg/L GAE, dan 30 menit adalah 177,2 mg/L GAE. Uji One Way ANOVA menunjukkan adanya perbedaan kandungan total fenol dalam air rebusan akar alang-alang pada berbagai waktu perebusan. Uji Beda Nyata Terkecil (BNT) menunjukkan adanya perbedaan kadar fenol total yang nyata pada setiap waktu perebusan. Penelitian ini menyimpulkan bahwa terdapat perbedaan kadar fenol total pada air rebusan akar alang-alang berdasarkan waktu perebusan.
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