Program Kreativitas Mahasiswa - Penelitian
PKM-P 2013

IDENTIFIKASI MUTASI GEN rpoB PADA ISOLAT Mycobacterium tuberculosis MULTIDRUG RESISTANT DENGAN METODE NESTED POLYMERASE CHAIN REACTION

Wijaya, Made Dharmesti ( Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam)
Rusyanthini, Eka Putri ( Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam)
Pradnyaniti, Desak Gede ( Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam)
Komalasari, Komang Trisna ( Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam)
Sunarti, Luh Putu Suci Sri ( Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam)

Article Info

Publish Date
28 Dec 2013

Abstract

      The rpoB gene has been known to be a surrogate marker for detection of Multidrug Resistant Tuberculosis (MDR-TB). Mutations of rpoB gene were associated with rifampicin (RMP) resistance occur in 81 bp region called Rifampin Resistance-Determining Region (RRDR). This study was aimed to determine the types of mutations and amino acid differences in isolates of M.tuberculosis MDR compared to M.tuberculosis H37Rv. Amplification of rpoB gene fragments was carried out using nested Polymerase Chain Reaction (nested PCR). Analysis of mutation using MEGA4 showed two amino acid changes namely, E418D and H526L. The type of mutations determined in isolate was missense mutations. Keywords: MDR-TB, mutation, nested PCR, rpoB

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Program Kreativitas Mahasiswa - Penelitian
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DITJEN DIKTI KEMDIKBUD RI

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