Biogenesis: Jurnal Ilmiah Biologi
Vol 1, No 1 (2013)

Analisis Variasi Genetik Melon (Cucumis melo L.) Kultivar Gama Melon Basket Dengan Metode Random Amplified Polymorphic DNA

Nur Huda, Ikhsan ( Laboratorium Genetika, Fakultas Biologi, Universitas Gadjah Mada)
Setiadi Daryono, Budi ( Laboratorium Genetika, Fakultas Biologi, Universitas Gadjah Mada)



Article Info

Publish Date
03 Jun 2015

Abstract

Melon (Cucumis melo L.) cultivars Gama Melon Basket (GMB) is the result of plant breeding that has the potential to be a product of local melon seed in Indonesia. This cultivar phenotype characters was known, but its genetic variation is not further studied. This study aimed to analyze the genetic variation of melon cultivars GMB and compared with 13 commercial melons and four members of the family Cucurbitaceae such as; cucumber, cucumber queen, barteh, and cantaloupe (blewah). RAPD-PCR method with 11 random primer was used to evaluate genetic variation in melon. Level of genetic similarity was determined by the formula simple matching coefficient (SSM) and the dendogram constructed by clustering analysis unweighted pair-group using arithmeticaverages (UPGMA). Results of RAPD-PCR with 11 primer produced 165 loci and it contains 84.85 % polymorphic DNA and 15.15 % monomorphic DNA. Results of genetic variation analysis suggests that 15 commercial melons differ from groups of cucumber, cucumber queen, barteh and cantaloupe(blewah). Cultivars GMB has genetic marker that can be distinguished from other commercial melons. GMB cultivar have specific polymorphic DNA on the size of 285 bp generated by PMAR primer.Keywords: Cucumis melo L., Gama Melon Basket, genetic variation, RAPD

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Journal Info

Abbrev

biogenesis

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic ...