JURNAL ILMIAH PETERNAKAN TERPADU
Vol 10, No 3 (2022)

Identification and Pathogenicity of Escherichia coli from Cloacal Swabs

Arie Khoiriyah (Universitas Lampung)
Sumardi Sumardi (Universitas Lampung)
Hendri Busman (Universitas Lampung)



Article Info

Publish Date
30 Jan 2023

Abstract

Colibacillosisis is an infectious disease in poultry caused by pathogenic Escherichia coli (E. coli) as primary or secondary agents. This study were aim to identify and test the pathogenicity ofE. coli isolated from chicken cloacal swabs. The samples used in this study were E. coli isolated from chicken cloacal swabs as many as 42 samples. The first stage in this research was subculturing E.coli in Eosin Methylene Blue Agar (EMBA) medium, then identified by Gram stain and biochemistry with Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA) and Methyl Red Voges-Proskauer (MRVP). Pathogenicity of E. coli was tested by culturing in Blood Agar to see the results of hemolysin production and on Sorbitol-MacConkey Agar (SMAC) to see the ability of Sorbitol fermentation. The results obtained were 12 isolates was pathogenic E. coli looked from the results of the pathogenicity test in the Blood Agar and SMAC Agar.

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Journal Info

Abbrev

JIPT

Publisher

Subject

Earth & Planetary Sciences Other

Description

JURNAL ILMIAH PETERNAKAN TERPADU (JIPT) is a double blind peer-reviewed open-access journal with an editorial board made up of experts in this field. JIPT is a scientific journal covering various aspects of animal husbandry science published since 2012. JIPT is published three times a year in March, ...