Jambura Journal of Health Sciences and Research
Vol 5, No 1 (2023): JANUARI: JAMBURA JOURNAL OF HEALTH SCIENCES AND RESEARCH

ANTIOXIDANT EFFECTIVENESS TEST USING UV-VISIBLE SPECTROPHOTOMETRY IN FORMULATIONS ETHANOL EXTRACT OF KELUBI FRUIT FLESH (Eleiodoxa conferta (Griff.) Burret) MOISTURIZING CREAM PREPARATIONS

Kanne Dachi (Universitas Tjut Nyak dhien)
Sudewi Sudewi (Unknown)
Nilsya Febrika Zebua (Unknown)
Siti Salmiyah (Unknown)
Ika Julianti Tambunan (Unknown)



Article Info

Publish Date
26 Jan 2023

Abstract

Buah kelubi (Eleiodoxa conferta (Griff.) Burret)  adalah tanaman buah yang mengandung senyawa flavonoid sangat bermanfaat untuk kulit. Kebaruan penelitian ini karena peneliti melakukan pengujian efektivitas antioksidan menggunakan spektrofotometri uv-visible pada formulasi ekstrak etanol daging buah kelubi dalam sediaan krim pelembab kulit. Tujuan penelitian untuk menganalisis ekstrak etanol daging buah kelubi yang diformulasikan kedalam sediaan krim pelembab pada beberapa variasi konsentrasi yang memiliki aktivitas sebagai antioksidan dan tidak menimbulkan iritasi pada kulit. Penelitian menggunakan metode deskriptif dan eksperimental dengan bahan uji daging buah kelubi yang di maserasi menggunakan penyari etanol 96% diformulasikan kedalam krim pelembab menggunakan konsentrasi 1,5%, 2,0%, 2,5% serta blanko. Hasil pengujian pada sediaan krim pelembab menunjukkan bahwa ekstrak etanol daging buah kelubi (Eleiodoxaconferta (Griff.) Burret) dapat diformulasikan dalam bentuk sediaan krim pelembab yang homogen dengan tipe emulsi M/A serta mempunyai nilai pH yang sesuai denga pH kulit fisiologis manusia yaitu nilai pH sediaan krim pelembab sesaat setelah dibuat adalah 6,1-6,5 dan pH sediaan krim pelembab setelah dilakukan Cycling test adalah 6,0-6,3. Sediaan krim pelembab dengan konsentrasi ekstrak etanol daging buah kelubi sebesar 2,5% adalah sediaan terbaik yang mampu memberikan kelembaban pada kulit sukarelawan dengan nilai 56 dimana nilai tersebut dikategorikan  “Hidrasi”, dengan persen pemulihan kelembapan pada kulit sebesar 28,04% selama 4 minggu pemakaian dengan kategori antioksidan sedang (IC50= 118,4903 ppm) dan blanko tergolong sebagai antioksidan yang sangat lemah (IC50= 243,0549 ppm). Kesimpulannya sediaan krim pelembab ekstrak etanol daging buah kelubi tidak menimbulakan efek mengiritasi kulit.Kata Kunci: Antioksidan; Ekstrak etanol; Buah kelubi; Krim pelembab.AbstractKelubi fruit (Eleiodoxa conferta (Griff.) Burret) is a fruit plant that contains flavonoid compounds that are very beneficial for the skin. The novelty of this study is that researchers tested the effectiveness of antioxidants using UV-visible spectrophotometry on the formulation of ethanol extract of kelubi fruit flesh in skin moisturizing cream preparations. The purpose of the study was to analyze ethanol extract of kelubi fruit flesh formulated into a moisturizing cream preparation at several concentration variations that have activity as antioxidants and do not cause irritation to the skin. The study used descriptive and experimental methods with test materials for kelubi fruit flesh which was macerated using 96% ethanol filtering formulated into a moisturizing cream utilizing a concentration of 1.5%, 2.0%, 2.5%, and blanks. The test results on moisturizing cream preparations showed that ethanol extract of kelubi fruit flesh (Eleiodoxaconferta (Griff.) Burret) can be formulated in the dosage form of a homogeneous moisturizing cream with an M / A emulsion type and has a pH value that is under the pH of human physiological skin, namely the pH value of the moisturizing cream preparation shortly after it is made is 6.1-6.5, and the pH of the moisturizing cream preparation after the cycling test is 6.0-6.3. A moisturizing cream preparation with a concentration of ethanol extract of kelubi fruit flesh 2.5% is the best preparation that can provide moisture to the skin of volunteers with a value of 56 where the value is categorized as "Hydration," with a moisture recovery percent on the skin of 28.04% for 4 weeks of use with a moderate antioxidant category (IC50 = 118.4903 ppm) and blanks classified as very weak antioxidants (IC50 = 243.0549 ppm). In conclusion, preparing the moisturizing cream of ethanol extract from kelubi fruit flesh does not cause an irritating effect on the skin.

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Journal Info

Abbrev

jjhsr

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Nursing Public Health

Description

Jambura Journal of Health Sciences and Research is a health scientific journal which published original articles of public health. This articles Published Twice a year in January and July. Which Focus and Scope in public health issues, including : Epidemiology, Health Education and Promotion, ...