Journal of Health Sciences and Medical Development
Vol. 2 No. 01 (2023): Journal of Health Sciences and Medical Development

Prevalence of CTX-M Gene as an ESBL Marker in Clinical Klebsiella pneumoniae Isolates

Arafah Nurfadillah (Universitas Megarezky, Indonesia)
Wahdaniar (Universitas Megarezky, Indonesia)
Ummu Syauqah Al Musyahadah (Universitas Megarezky, Indonesia)
Nur Insani Amir (Universitas Megarezky, Indonesia)
Ade Irma (Universitas Megarezky, Indonesia)
Miladiarsi (Universitas Megarezky, Indonesia)
Tenri Ayu Adri (Universitas Megarezky, Indonesia)

Article Info

Publish Date
01 Feb 2023

Abstract

Klebsiella pneumonia is a bacteria that causes many negative impacts on health, primarily due to its tendency to develop antibiotic resistance. This research aims to identify the prevalence of the CTX-M gene as an ESBL marker of the Clinical Isolate of K. pneumoniae of Wahidin Sudirohusodo General Hospital. The samples consisted of 50 Clinic isolates of K. pneumoniae which fulfilled the inclusion and exclusion criteria. The research procedure consisted of DNA extraction, amplification, and agarose gel electrophoresis. The research results indicate that 38 samples (76%) are positive for the CTX-M gene, while 12 samples (24%) are negative or not found CTX-M gene. Thus, the prevalence of the CTX-M gene in K. pneumoniae resistant to beta-lactam antibiotics in Wahidin Sudirohusodo General Hospital is relatively high.

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Journal Info
Journal of Health Sciences and Medical Development
Website

Abbrev

HESMED

Publisher

Indonesian Institute of Science and Technology Research

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Neuroscience Veterinary

Description

Journal of Health Sciences and Medical Development is an international peer-reviewed journal publishing original and high-quality papers in all aspects of the science and practice of public health. As an academic exchange media, scientists and researchers can know the up-to-date trends and seek ...