The Journal of Experimental Life Sciences (JELS)
Vol. 13 No. 1 (2023)

Isolation of Chitinolytic Bacteria as Biocontrol Agent for Pathogenic Fungi on Cocoa Fruit in Sumbermanjing District, Malang

Cahyaning Sulistyantini (Brawijaya University)
Irfan Mustafa (Universitas Brawijaya)
Yoga Dwi Jatmiko (Universitas Brawijaya)



Article Info

Publish Date
28 Feb 2023

Abstract

The productivity of cocoa plants in Sumbermanjing District is lower than in other parts of Malang Regency. Diseases like pathogenic fungi are to blame for the low output of cocoa plants. This research aimed to find chitinolytic bacteria that were superior at degrading chitin and limiting the growth of pathogenic fungi. These bacteria were isolated from cocoa garden soil and shrimp shell dumps. The bacteria from soil samples taken from the shrimp shell dump in Ujungpangkah District and the cocoa gardens in Sumbermanjing District were cultivated on an agar medium supplemented with colloidal chitin. The pathogenic fungi were discovered from decaying cocoa pods from Sumbermanjing District cocoa plantations. An antagonist test was used to gauge the inhibitory power, and a semi-quantitative test was used to measure the chitinolytic index. The similarity of 16S rDNA sequences allowed for the identification of superior bacterial isolates. The T2S1 isolates of chitinolytic bacteria had the highest levels of pathogenic fungi inhibition (25.14%, 20.85%, and 25.24%). With a 16S rDNA sequence similarity of 99.77%, Bacillus subtilis C10 was identified as the bacterium T2S1. Keywords: Bacillus subtilis, Pathogenetic fungi, Cocoa, Chitinase, 16s-rDNA.

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Journal Info

Abbrev

jels

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

Description

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific ...