Jurnal Medik Veteriner
Vol. 6 No. 1 (2023): April

Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences

Muhammad Fajar Amrullah (Master of Reproductive Biology, Faculty of Veterinary Medicine, Universitas Airlangga)
Budi Utomo (Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga)
Suzanita Utama (Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga)
Tri Wahyu Suprayogi (Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga)
Tita Damayanti Lestari (Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga)
Tjuk Imam Restiadi (Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga)
Ristaqul Husna Belgania (Master of Reproductive Biology, Faculty of Veterinary Medicine, Universitas Airlangga)

Article Info

Publish Date
01 Apr 2023

Abstract

The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.

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Journal Info
Jurnal Medik Veteriner
Website

Abbrev

JMV

Publisher

Universitas Airlangga

Subject

Biochemistry, Genetics & Molecular Biology Veterinary

Description

urnal Medik Veteriner (JMV) publishes high quality and novelty papers focusing on Veterinary and Animal Science. The fields of study are anatomy, pathology, basic medicine, veterinary public health, microbiology, veterinary reproduction, parasitology, animal husbandry and animal welfare. Food ...