Jurnal Kedokteran Hewan
Vol 14, No 3 (2020): September

THE POTENTIAL OF ADJUVANT AGAINST PRODUCTION OF ANTISTREPTOCOCCAL IMMUNOGLOBULIN Y (IGY) IN AQUACULTURE

Rifky Rizkiantino (Division of Microbiology Medic, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia)
I Wayan Teguh Wibawan (Bogor AgriculturaDivision of Microbiology Medic, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia l University (IPB University))
Fachriyan Hasmi Pasaribu (Division of Microbiology Medic, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia)
Retno Damajanti Soejoedono (Division of Microbiology Medic, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia)
Okti Nadia Poetri (Division of Microbiology Medic, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia)
Wyanda Arnafia (Division of Research and Development, PT. Tekad Mandiri Citra, Bandung, Indonesia)
Kris Damar Sasi (Division of Research and Development, PT. Tekad Mandiri Citra, Bandung, Indonesia)
Dinda Reisinta (Division of Research and Development, PT. Tekad Mandiri Citra, Bandung, Indonesia)



Article Info

Publish Date
19 Sep 2020

Abstract

This study was conducted to explore the potential of adjuvant for the production of immunoglobulin Y (IgY) as antistreptococcosis in layer chicken with mass production orientation. Enterococcus faecalis which causes streptococcosis in the red tilapia was selected as a candidateantigen. The production of immunoglobulin Y (IgY) was carried out on Isa Brown layer chickens and aged around 20 weeks. Furthermore, thechickens were grouped into four groups (A, B, C, and D groups), each consisting of three chickens based on the type of adjuvant, while twochickens were used as a control group. Each group was treated by giving MONTANIDE™ ISA 71R VG adjuvant (A), Freund's adjuvant (B), aluminum potassium sulphate adjuvant (KAl(SO4)2∙12H2O) concentration of 50 ppm in pH 7 (C), and only antigens without adjuvant (D). Chickens were kept for 35 days and each week was checked for presence the IgY antigen in the serum and egg yolk. Booster was conducted on 14th and 28th days of maintenance. The results showed that IgY in treatment group A was detected on day 28 in the serum and day 35 in the yolk. Whereas the treatment group B could be detected on day 35 in the serum. However, the IgY was not detected in the serum and yolk in C, D, and control groups until the end of the maintenance. Based on the results, it can be concluded that the appearance of IgY in serum and yolk in a relatively fast time is obtained in the combination of Enterococcus faecalis antigen with the emulsion of water-in-oil adjuvant (SEPPICMONTANIDE™ ISA 71R VG) compared to the other types of adjuvant that use in this study.

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Journal Info

Abbrev

JKH

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian ...