Jurnal Pertanian Agros
Vol 25, No 2 (2023): edisi April

SELEKSI DAN KARAKTERISASI PLANLET BAWANG MERAH (Allium ascalonicum L.) TERHADAP CEKAMAN GARAM NATRIUM KLORIDA (NaCl) SECARA IN VITRO

Herlina Putri Prastiwi (Unknown)
Endang Nurcahyani (Unknown)
Tundjung Tripeni Handayani (Unknown)
Sri Wahyuningsih (Unknown)



Article Info

Publish Date
02 Jul 2023

Abstract

Onion (Allium ascalonicum L.) is a spice commodity that has many benefits and high economic value. One of the factors causing low onion production is due to less productive land due to salt stress or salinity. This study aims to determine the specific character and effective concentration of NaCl for selection of onion plantlets under salt stress conditions in vitro. The research design was in the form of a completely randomized design (CRD) consisting of 1 factor, namely NaCl concentration: 0%, 0.15%, 0.30%, 0.45%, 0.60% with 5 replications. The homogeneity of the data was analyzed by Levene's test and continued with the oneway Anova test and Tukey's test at 5% significance level. The results showed that the effective concentration of NaCl for selection of onion plantlets under salt stress conditions in vitro was 0.15%. The higher the concentration of NaCl, the smaller the size of the plantlets with the lower the height of the plantlets, the visualization of the leaf color becomes yellowish green to brown, and the decreased content of chlorophyll a, b, and total chlorophyll. INTISARIBawang merah (Allium ascalonicum L.) merupakan salah satu komoditas rempah yang memiliki banyak manfaat dan bernilai ekonomi tinggi. Salah satu faktor penyebab rendahnya produksi bawang merah karena adanya lahan kurang produktif akibat cekaman garam atau salinitas. Penelitian ini bertujuan untuk mengetahui konsentrasi NaCl yang efektif untuk seleksi dan karakterisasi planlet bawang merah dalam kondisi cekaman garam secara in vitro. Rancangan penelitian berupa Rancangan Acak Lengkap (RAL) yang terdiri dari 1 faktor yaitu konsentrasi NaCl: 0%, 0.15%, 0.30%, 0.45%, 0.60% dengan 5 kali ulangan. Homogenitas data dianalisis dengan uji Levene dan dilanjutkan dengan uji Anova oneway dan Uji Tukey pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa konsentrasi NaCl yang efektif untuk seleksi planlet bawang merah dalam kondisi cekaman garam secara in vitro adalah 0,15%. Semakin tinggi konsentrasi NaCl, maka ukuran planlet semakin kecil dengan tinggi planlet yang semakin rendah, visualisasi warna daun menjadi hijau kekuningan hingga cokelat, serta kandungan klorofil a, b, dan klorofil total yang semakin menurun.

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Journal Info

Abbrev

JA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Pertanian Agros (JPA) is published by Faculty of Agriculture, Janabadra University and the Agribusiness Association of Indonesia (AAI). It available online supported by Directorate General of Higher Education - Ministry of Research, Technology, and Higher Education- Republic of Indonesia JPA ...