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Efek Ekstrak Metanol Makroalga Cokelat (Sargassum sp.), Merah (Gracillaria sp.) dan Taurin Terhadap Gambaran Histopatologi Hepar Mencit Jantan (Mus musculus) yang Diinduksi Benzo(?)Piren Hervidea, Radella; Widiastuti, Endang Linirin; Nurcahyani, Endang; Sutyarso, Sutyarso; Susanto, G. Nugroho
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3670

Abstract

ABSTRACTCancer is a disease marked by the existence of abnormal growth of cell continuously and uncontroll in an organism body. Benzo(?)pyrene is one of the substances that can cause a cancer. The methanolic extract of Sargassum sp. and Gracillaria sp. has anticancer and antioxidant activity. Other compounds with antioxidant activity is taurine. The aim of this research is to examine the effect from methanolic extract of Sargassum sp. and Gracillaria sp .and also taurine on hepar histopatology of male mice (Mus musculus) induced by benzo(a)pyrene. This research used Completely Randomized Design. 30 male mice were divided into 6 groups of treatments, those were: K1 = (K-), K2= induced by benzo(?)pyrene for 10 days, K3= after induced by benzo(?)pyrene, were given the Sargassum sp. orally with a dose 8mg/mice during 15 days, K4=after induced by benzo(a)pyrene, were given the combination of Sargassum sp. (8mg/mice) and taurine(15,6mg/mice) orally during 15 days, K5= after induced by benzo (?)pyrene, were given the Gracillaria sp.orally with a dose 8mg/mice during 15 days, and K6=after induced by benzo(a)pyrene, were given the combination of Gracillaria sp. (8mg/mice) and taurine (15,6mg/mice) orally during 15 days. Data were analyzed using ANOVA (? = 5%). The result shows that the combination of Sargassum sp. and Gracillaria sp. and taurine can increase the number of erythrocyte , decrease the number of leucocyte and protect the damage of hepar histopatology of male mice (Mus musculus) induced by benzo (?)pyrene. Combination of extract Gracillaria sp. and taurine is the most effective to protect the damage hepar histopatology of male mice(Mus musculus) induced by benzo(? )pyrene.Keywords: male mice,Sargassum sp., Gracillaria sp., taurine, benzo(a)pyrene, hepar
Uji Ketahanan Planlet Anggrek Bulan (Phalaenopsis amabilis (L.) Bl.) Hasil Seleksi dengan Asam Salisilat Terhadap Fusarium oxysporum Secara In Vitro Noviantia, Ria Aulia; Nurcahyani, Endang; Lande, Martha Lulus
Jurnal Penelitian Pertanian Terapan Vol 17, No 2 (2017)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.099 KB) | DOI: 10.25181/jppt.v17i2.292

Abstract

Phalaenopsis amabilis cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum. Plantlet P.amabilis which resistant to F. Oxysporum was selected in the solid Vacin and Went (VW) medium was added with salicylic acid at concentrations of 65 ppm, 75 ppm, and 85 ppm, compared with controls (0 ppm). The goals of the research were to study and determine of: 1) The SA concentration of planlet P. amabilis selection tolerant; 2) The proper concentration of AS during in vitro selection for suppressing the Fo. The research was carried out in December 2015 to February 2016 in the Botany Laboratory (in vitro research room), Departement of Biology, Faculty of MIPA, Lampung University. The result showed that: 1) The SA tolerant concentration for plantlet selection with P. amabilis was between 65 ppm - 85 ppm. 2) The 85 ppm of SA was effective for suppressing the Fo compared to 65 ppm and 75 ppm. Keywords: Phalaenopsis amabilis, salicylic acid, Fusarium oxysporum, In vitro, Resistance.
Aktivitas Enzim Peroksidase Daun Planlet Pisang Ketan (Musa paradisiaca L.) Hasil Pengimbasan Ketahanan terhadap Asam Salisilat secara In Vitro Muslimah, Imamah; Nurcahyani, Endang; Zulkifli, Zulkifli
Jurnal Penelitian Pertanian Terapan Vol 17, No 2 (2017)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.827 KB) | DOI: 10.25181/jppt.v17i2.288

Abstract

Banana plant production are decreased because it getting an attack from Fusarium oxysporum. ?ketan? Banana plantlet that resistant to the Fusarium oxysporum were selected by in vitro in the solid Murashige and Skoog (MS) medium with added salicylic acid at 40 ppm, 50 ppm, 60 ppm, and 70 ppm concentration, compared with controls (0 ppm). The aim of this research is to determine the activity of peroxidase enzyme on leaves of banana ketan plantlet. The research was carried out in December 2015 to February 2016 in the Laboratory of Botani (in vitro room), Departement of Biology, Faculty of MIPA, Lampung University. This study used a completely randomized design with 5 replications. Data were analyzed with the variance (Anova) and if them different will be continued by LSD test performed at 5% significance level. Extraction of peroxidase method using Saravanan et al. (2004) with the mixture of pyrogallol and ?2?2. The absorbance was measured with a spectrophotometer (Shimudzu UV 800) at wavelength (?) of 420 nm and readable from zero. The result showed that the activity of peroxidase enzyme on the leaves banana ketan plantlet has increased optimally in the salicylic acid concentration of 50 ppm compared with controls. Increased of Peroxidase enzyme activity showed that banana ketan plantlet was resistant to the salicylic acid and expected to be resistant to Fusarium oxysporum. Keywords: Salicylic acid, Peroxidase enzyme, Fusarium oxysporum, in vitro, Banana ketan
EFEKTIVITAS EKSTRAK DAGING BUAH NANAS (ANANAS COMOSUS L.) DALAM PENURUNAN INDEKS BROWNING DARI UMBI KENTANG (SOLANUM TUBEROSUM L.) Wardanis, Putri; Zulkifli, Zulkifli; Lande, Martha L.; Nurcahyani, Endang
Jurnal Penelitian Pertanian Terapan Vol 19, No 2 (2019)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v19i2.1568

Abstract

The purpose of this study was to determine whether the pineapple fruit extract can inhibit theprocess of browning on the potato tubers. This study used Completely Randomized Design(RAL) with 5 levels of pineapple fruit concentration of 0% v/v, 25% v/v. 50% v/v, 75% v/v,and 100% v/v and consist of 5 replications. Qualitative Parameters in this study was the colosurface of potato tubers while quantitative parameters were browning index, total soluble carbohydrate content, and dehydrogenase enzyme activity homogeneity of variance, analysisof variance, and Tukey test were conducted at 5% significant level. Correlations betweendependent and independent variables were determined by linear regression. The resultshowed that the color surface of potato tuber treated with the concentration of pineappleextract 100% v/v was less brown than control and other concentrations. Index browning ofpotato tuber treated with concentration 75% v/v and 100% v/v was significantly decreased. Concentration of pineapple extract was negative linearly correlated to browning index ofpotato tubers. The concentration of pineapple extract 100% v/v increased significantly totalsoluble carbohydrate of potato tubers. The concentration of pineapple extract was positivelinearly correlated to total soluble carbohydrate. The activity of dehydrogenase enzyme ofpotato tuber treated with concentration 100% v/v was significantly increased. Theconcentration of pineapple extract was quadratic correlated to the activity of dehydrogenaseenzyme.
EFEK EKSTRAK METANOL MAKROALGA COKELAT (SARGASSUM SP.), MERAH (GRACILLARIA SP.) DAN TAURIN TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT JANTAN (MUS MUSCULUS) YANG DIINDUKSI BENZO(?)PIREN Hervidea, Radella; Widiastuti, Endang Linirin; Nurcahyani, Endang; Sutyarso, Sutyarso; Susanto, G. Nugroho
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3670

Abstract

ABSTRACTCancer is a disease marked by the existence of abnormal growth of cell continuously and uncontroll in an organism body. Benzo(?)pyrene is one of the substances that can cause a cancer. The methanolic extract of Sargassum sp. and Gracillaria sp. has anticancer and antioxidant activity. Other compounds with antioxidant activity is taurine. The aim of this research is to examine the effect from methanolic extract of Sargassum sp. and Gracillaria sp .and also taurine on hepar histopatology of male mice (Mus musculus) induced by benzo(a)pyrene. This research used Completely Randomized Design. 30 male mice were divided into 6 groups of treatments, those were: K1 = (K-), K2= induced by benzo(?)pyrene for 10 days, K3= after induced by benzo(?)pyrene, were given the Sargassum sp. orally with a dose 8mg/mice during 15 days, K4=after induced by benzo(a)pyrene, were given the combination of Sargassum sp. (8mg/mice) and taurine(15,6mg/mice) orally during 15 days, K5= after induced by benzo (?)pyrene, were given the Gracillaria sp.orally with a dose 8mg/mice during 15 days, and K6=after induced by benzo(a)pyrene, were given the combination of Gracillaria sp. (8mg/mice) and taurine (15,6mg/mice) orally during 15 days. Data were analyzed using ANOVA (? = 5%). The result shows that the combination of Sargassum sp. and Gracillaria sp. and taurine can increase the number of erythrocyte , decrease the number of leucocyte and protect the damage of hepar histopatology of male mice (Mus musculus) induced by benzo (?)pyrene. Combination of extract Gracillaria sp. and taurine is the most effective to protect the damage hepar histopatology of male mice(Mus musculus) induced by benzo(? )pyrene.Keywords: male mice,Sargassum sp., Gracillaria sp., taurine, benzo(a)pyrene, hepar
Efektivitas Pemberian Pupuk Organik Cair dari Tiga Jenis Rebung Bambu Terhadap Pertumbuhan Tanaman Tomat (Solanum lycopersicum Mill.) Alfian Bagus Setiawan; Yulianty Yulianty; Endang Nurcahyani; Martha Lulus Lande
Biosfer: Jurnal Tadris Biologi Vol 10, No 2 (2019): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.4 KB) | DOI: 10.24042/biosfer.v10i2.4591

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui efek dari pemberian pupuk organik cair rebung bambu terhadap pertumbuhan tanaman tomat (Solanum lycopersicum Mill.) dan mengetahui konsentrasi yang paling baik untuk pertumbuhannya. Penelitian ini telah dilaksanakan pada bulan Januari 2018 sampai bulan Februari 2018. Penelitian dilakukan di laboratorium lapang terpadu, Fakultas Pertanian Universitas Lampung dan laboratorium botani Fakultas Matematika dan Ilmu Pengetahuan Alam. Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 16 perlakuan. Rebung Bambu Betung (Dendrocalamus asper (Schult) Backer e.x Heyne), rebung Bambu Tali (Gigantochloa apus (Schulft.f.) Kurz.), dan rebung Bambu Kuning (Bambusa Vulgaris) dengan konsentrasi pupuk organik cair masing-masing jenis bambu terdiri dari 6 konsentrasi yaitu : kontrol (0%), 5%, 10%, 15%, 20%, dan 25%, dilakukan ulangan sebanyak 2 kali. Analisis data menggunakan langkah-langkah yaitu uji homogenitas menggunakan uji levene dengan taraf 5% dan uji ragam (ANOVA). Hasil penelitian menunjukkan perlakuan pupuk organik cair rebung bambu betung dengan konsentrasi 10% (A1B2) perlakuan yang terbaik terhadap tinggi tanaman, panjang akar, berat kering, kandungan klorofil a dan kandungan klorofil b, namun tidak berpengaruh terhadap kandungan klorofil total tanaman tomat.
Uji Potensi Bioherbisida Ekstrak Daun Mahoni (Swietenia mahagoni (L.) Jacq) Terhadap Pertumbuhan Gulma Maman Ungu (Cleome rutidosperma D.C.) Agung Kurniawan; Yulianty Yulianty; Endang Nurcahyani
Biosfer: Jurnal Tadris Biologi Vol 10, No 1 (2019): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.213 KB) | DOI: 10.24042/biosfer.v10i1.4232

Abstract

Penelitian ini bertujuan untuk menguji ekstrak daun mahoni (Swietenia mahagoni (L.) Jacq.) terhadap pertumbuhan gulma maman ungu (Cleome rutidosperma D.C.). Penelitian menggunakan metode RAL (Rancangan Acak Lengkap) dengan konsentrasi 0%, 10%, 20%, 30%, 40%, 50%, dan 60% dengan ulangan sebanyak 4 kali. Variabel dalam penelitian ini adalah tinggi tanaman, berat basah, jumlah helai daun, kandungan klorofil a, kandungan klorofil b, dan kandungan klorofil total. Uji homogenitas dilakukan menggunakan uji Bartlett, selanjutnya dianalisis menggunakan metode statistik ANOVA (Analysis of Variance) pada taraf nyata 5% (p 0,05) jika terdapat beda nyata dilanjutkan dengan uji BNT pada taraf nyata 5%. Hasil penelitian menunjukkan ekstrak daun mahoni dengan konsentrasi 10% efektif untuk menghambat pertumbuhan tinggi dan jumlah helai daun, sedangkan konsentrasi 20% efektif untuk menghambat berat basah gulma maman ungu. Semakin tinggi konsentrasi yang digunakan maka semakin efektif ekstrak daun mahoni untuk menghambat pertumbuhan gulma maman ungu. Ekstrak daun mahoni belum mempengaruhi kadar klorofil gulma maman ungu.
Morphological Characteristics of Araceae Plants in Liwa Botanical Garden, West Lampung Risa Suryani Wilyasari; Yulianty Yulianty; Zulkifli Zulkifli; Endang Nurcahyani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 1 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i1.13

Abstract

Araceae is one type of plant that has a fairly high level of diversity and also rich in benefits such as a source of food, ornamental plants, and medicine. Research on the characteristics of Araceae in Indonesia is still limited. The purpose of this study was to identify Araceaeplants based on morphological structure and analyze the morphological characteristics ofAraceae in Liwa Botanical Garden, West Lampung. This research was conducted from November 2019 to January 2020. Data were collected at Liwa Botanical Garden, WestLampung based on exploration and observation collection methods. Morphological structure data were analyzed as descriptive and qualitative. The parts of plants that are compared between Araceae plants are the shape, size of leaves, petiole, and inflorescences. Observations show that 21 genera of Araceae are consisting of 26 plants. Araceae plants have three different ways of life, namely terrestrial, epiphytic, and aquatic. The shape of the leaves is ovatus, cordatus, lanceolatus, cuneatus, needle, peltatus, sagittatus, hastatus, palmatisect, bipinnatisect and tripinnatisect. The characteristics of this plant have compound interest with cob types (spadix) which are covered by a spatha with two types of inflorescences, namely unisexual and bisexual.
Stomata Index of Cattleya sp. Lindl., Planlet in Drought-Stress Conditions Tara Sesafia Paletri; Endang Nurcahyani; Yulianty Yulianty; Rochmah Agustrina
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.23

Abstract

Cattleya was one of the most popular orchids by Indonesian. Constraints in cultivating Cattleya included the lack of water availability in an area, causing drought stress. Stress in drought in vitro can be simulated by adding Poly Ethylene Glycol 6000 and growth regulators in atonic form. The purpose was to determine the effect of atonic solution and PEG 6000 on the stomata index toward in vitro drought stress conditions. This study was carried out in a 3 x 3 factorial experiment. Factor A was an atonic solution with 3 concentration levels: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v, 25% b/v. The results showed that the higher the concentration of PEG 6000 and atonic concentrations caused a decrease in the stomata index of Cattleya orchid plantlets under drought stress conditions in vitro.
Nepenthes mirabilis (Lour.) Druce Planlet at a Various Levels of Murashige & Skoog Medium Density In Vitro Nadia Fakhriyati Arfa; Endang Nurcahyani; Zulkifli Zulkifli; Tundjung Tripeni Handayani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.25

Abstract

This study aims to determine the variation of the stomata index of the Kantong Semar (Nepenthes mirabilis) planlet at a various medium density of the Murashige and Skoog. This study used a Completely Randomized Design using one factor (medium density of the Murashige and Skoog). We used 5 levels of medium density, i.e.: 1/16 MS, 1/8 MS, 1/4 MS, 1/2 MS, and MS. Homogeneity test used Levene’s test of 5% significance level, then analysis of variance is carried out at 5% significance level and followed by Tukey test at 5% significance level. The results showed that the lower the level of Murashige and Skoog medium density on the Nepenthes mirabilis plantlet, the stomata index also increased.
Co-Authors Adrianus Adrianus Agung Kurniawan Agustrina, Rochmah Alfian Bagus Setiawan Amirah Afifah Melta Anis Ashari Annindya Rahma Asrini Puspitasari Ayu Wulan Septitasari Ayu Azahra Putri Najla Bambang Hadisutrisno Bambang Irawan Bambang Irawan Bambang Irawan Damayanti, Elok Della Apriyanti Desti Deria Rahmadani Desti Syahfitri Dian Alfiah Dian Pratiwi Dwi Septiani E. Suharyanto Eko Nastiti Eko Pramono Elsi Diana Emantis Rosa Emantis Rosa Endang Linirin Eti Ernawiati Eti Ernawiati Eti Ernawiati Fesya Salma Putri Gardis Andari Hardoko Insan Qudus Hardoko Insan Qudus Hendri Busman Herlina Putri Prastiwi Ika Listiana Imamah Muslimah Imamah Muslimah, Imamah Indah Stellawati Intan Okta Nabilla Intan Okta Nabilla Issirep Sumardi Khairunnisa Rizqika A.P Kinasih Cahyono Lili Chrisnawati Lili Chrisnawati Lili Chrisnawati Linda Kurnia Dewi Linda Septiani Lindawati Lindawati Lucy Adi Tama M Kanedi Maemonah, Maemonah Mahfut Martha L. Lande Martha L. Lande Martha Lulus Lande Martha Lulus Lande Martha Lulus Lande Martha Lulus Lande, Martha Lulus Ma’ania Zalzabila Meilyana Santa Maria Meilyana Santa Maria Moza Fierda Atiek Muhamad Rizkci Sazilly Nadia Fakhriyati Arfa Nadya Rosyalina Nalindri Impitasari Ni Made Nada Elsika nuning nurcahyani Nur Aisyah Amini Nurul Aniqotun Mutmainah Primasari Linda Setiawati Putri Wardanis R. Fadly Bayu Dwiyoga Radella Hervidea, Radella Rahayu Amaliya Ratna Oktaviani Ratna Oktaviani Resta Tania Ria Aulia Noviantia Ria Aulia Noviantia, Ria Aulia Rina Maryani Risa Suryani Wilyasari Risma Rasmani Rochma Agustrina Rochmah Agustrina Rochmah Agustrina Sally Khoirunisa Sally Khoirunisa Salman Farisi Sarah Sarah Selvi Marcelia Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sumardi Sumardi Sumardi Sumardi, Sumardi Suratman Suratman Susanto, G. Nugroho Susanto, G. Nugroho Sutyarso Sutyarso Sutyarso Sutyarso Syavira Indriani T. Indah Setia Ningsih Tara Sesafia Paletri Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Wanda Amelia Wardanis, Putri Widiastuti, Endang Linirin Widiastuti, Endang Linirin Yulianti Yulianty Yulianty Soeradji Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zelfi Julita Dwi Putri Zulkifli Zulkifli Zulkifli Zulkifli Zulkifli Zulkifli Zulkifli zulkifli zulkifli Zulkifli Zulkifli Zulkifli Zulkifli