Fly-borne diseases include dysentery, cholera, abdominal typhus, diarrhea, and others related to poor environmental sanitation conditions, so it is necessary to control flies carefully to avoid environmental pollution and health problems. One plant that contains botanical insecticides is the fragrant pandanus leaf (Pandanus amaryllifolius). Fragrant pandanus leaves contain insecticides in the form of alkaloids, saponins, flavonoids, tannins, and polyphenols. The type of research conducted is experimental with a static group comparison experiment design, which simultaneously measures the dependent variable and the independent variable by comparing the treated results with the control results. The research sample amounted to 240 flies. In the 10-minute observation period, 20 flies were released in one cage for each treatment. The results of this study were obtained. There is a difference in the use of fragrant pandanus leaf extract with a dose of 5 ml (7.67), 10 ml (12.33), and 15 ml (13.67) and without using a dose of fragrant pandanus leaf extract on the level of house fly expulsion: 5 ml (2.33), 10 ml (1.67), and 15 ml (1.33). The dose that causes the most significant expulsion of houseflies is in the extract of fragrant pandanus leaves with a dose of 15 ml because the higher the dose, the more flies are expelled. Conclusion: The results of the ANOVA test based on the dose of fragrant pandanus leaf extract on the number of houses flies that do not land, the degree of freedom obtained from 3 to 8, are used to see Ft. It turns out that Ft 1% = 7.59, Ft 5% = 3.86, and Fo = 536.32; thus, if Fo is greater than Ft, Ho is rejected, meaning that there is a significant difference between the various doses of fragrant pandanus leaf extract on the repulsion of house flies.Â
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