Dental Journal (Majalah Kedokteran Gigi)
Vol. 56 No. 3 (2023): September

Amelogenin and alkaline phosphatase expression in ameloblast after saltwater fish consumption in pregnant mice (Mus musculus)

Sandy Christiono (Faculty of Dentistry, Universitas Islam Sultan Agung, Semarang, Indonesia)
Seno Pradopo (Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia)
I Ketut Sudiana (Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia)
Islamy Rahma Hutami (Faculty of Dentistry, Universitas Islam Sultan Agung, Semarang, Indonesia)
Regilia Shinta Mayangsari (Faculty of Dentistry, Universitas Islam Sultan Agung, Semarang, Indonesia)
Yayun Siti Rochmah (Faculty of Dentistry, Universitas Islam Sultan Agung, Semarang, Indonesia)
Zurairah Ibrahim (Faculty of Dentistry, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia)



Article Info

Publish Date
09 Jun 2023

Abstract

Background: The intricate process of tooth formation during embryonic development ensures sufficient nutrition for the growth of healthy dental tissues. Amelogenin and alkaline phosphatase (ALP) are serine proteinases secreted by the ameloblast during the transition and maturation phases of the amelogenesis process. Consumption of saltwater fish is predicted to increase the expression of amelogenin and ALP in ameloblast cells during tooth formation. Only now have the function of each gene, tooth-forming cells, and the proteins they produce in the biomolecular amelogenesis of tooth enamel, which began during prenatal development, been clarified. Purpose: This study aims to determine how saltwater fish powder affects the ability of mother mice to increase the expression of amelogenin and ALP in cell ameloblast. Methods: Using a completely randomized design, this study was experimental and aimed to examine the effects of sardine (Sardinella fimbriata), splendid ponyfish (Leiognathus splendens), and tuna (Euthynnus affinis) powder. As samples, twenty-four female mice (Mus musculus) were used. Two groups of mice were created: group 1 (2.14 mg/0.5 ml) and the control group. The expression of amelogenin and ALP was determined using immunohistochemistry (IHC) and t-test (p0.05). Results: Expression of ameloblast was significantly different between the treatment and control groups (p0.05). Conclusion: The consumption of saltwater fish reduces the amelogenin and ALP expressions of mouse fetal ameloblast cells during tooth development in vivo.

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Journal Info

Abbrev

MKG

Publisher

Subject

Dentistry

Description

The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly ...