Biosaintifika: Journal of Biology & Biology Education
Vol 15, No 2 (2023): August 2023

Development of Photomyxotrophic Culture Protocol of Lemongrass Through Sucrose Concentration Increase and Light Intensity Decrease

Desy Irmawati (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Kampus Sekaran, Gunungpati, Semarang, Indonesia 50229)
Enni Suwarsi Rahayu (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Kampus Sekaran, Gunungpati, Semarang, Indonesia 50229)



Article Info

Publish Date
15 Aug 2023

Abstract

Lemongrass (Cymbopogon nardus (L.) Randle) plantlets are interesting to be used as tissue culture-based souvenir. The success of plantlet propagation through photomyxotrophic culture is influenced by several factors, including sucrose concentration and light intensity. The research aimed to analyze the effect of sucrose concentration and light intensity on the growth of lemongrass plantlets. This experimental research used randomized block factorial design with two factors, namely sucrose concentration (0 g/l, 10 g/l, 20 g/l, 30 g/l) and light intensity (2000 lux, 3000 lux, 4000 lux, 5000 lux). The variables observed were some parameters of growth and development of plantlet. The data were analyzed with two-way Analyses of Variance and Duncan Multiple Range Test. The results showed that sucrose concentration had significant effect on the number of shoots and leaves of plantlet. The light intensity and the interaction of both factors had also significant effect on the number of shoots and leaves. The most optimal treatments were sucrose of 20 g/l and light intensity of 3000 lux.  The results of this study innovate the tissue culture technique using photomyxotrophic system in order to produce sterile lemongrass plantlets in large quantities.

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Journal Info

Abbrev

biosaintifika

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological ...