Jurnal Veteriner Nusantara
Vol 3 No 2 (2020): Agustus 2020

MORFOLOGI ABNORMALITAS SPERMATOZOA BABI LANDRACE DAN BABI DUROC DENGAN PEWARNAAN CARBOFUCHSIN

Ewaldus F. Patmawan (Unknown)
Cynthia D Gaina (Universitas Nusa Cendana)
Nancy D. F. K. Foeh (Universitas Nusa Cendana)



Article Info

Publish Date
30 Aug 2020

Abstract

This research was aimed to defined the morphology of boar’s sperm and using carbofuchsin staining. The semen sample were collected with technique glove hand method from three healty Landrace and Duroc boars belong to UPT pembibitan Ternak Babi Dinas Peternakan Provinsi NTT. Semen were stained using Carbofucsin staining technique, morphology observation was evaluated by microscop on 500 cells for each sample. From macroscopic and microscopic evaluation the result showed that the Landrace boar’s semen volume was308.33±23.63 ml, the pH was 7,4 and the sperm concentration was 230±20x106 cell/mL. Macroscopic and microscopic evaluation the result showed that the Duroc boar’s semen volume was 213.33±41.63 ml, the pH was 7,4 and the sperm concentration was 455±198.30x106 cell/mL. The result of morphology observation in Landrace and Duroc boars showed that the normal sperm was 95.91±1.14% and94.02±2.76%. Abnormal sperm in Landrace and Duroc was4.13±1.11% dan 5.97±2.76%. The abnormality of theLandrace and Duroc sperm consist of narrow (0.07% and0,26%), acrosome defect (0,27% and 0,1%), pear shapered (0,49% and 0.18%), double head (0,07% and 0,04%), variable size (0.11% and 0,12%), lose abnormal head (0.06% and 0,32%) and tail abnormalities (1,72% dan 4,36%). Based on the evaluation of semen and morphological observations in this research can be concluded that the boars semen used in good conditio

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Journal Info

Abbrev

jvn

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Public Health Veterinary

Description

Jurnal Veteriner is a peer-reviewed and open access journal that focuses on promoting veterinary sciences generated from basic sciences, clinical, and community or public health research to integrate researches in all aspects of animal health toward human prosperity. This journal publishes original ...