Kenanga : Journal of Biological sciences and Applied Biology
Vol 3 No 1 (2023): April 2023

POTENSI ENZIM SELULASE DARI BAKTERI TERMOFILIK DI KAWASAN WISATA IE SUUM KABUPATEN ACEH BESAR

Rahmatil Majidah (Program Studi Biologi, Fakultas Sains dan Teknologi, UIN Ar-Raniry Banda Aceh)
Syafrina Sari Lubis (Program Studi Biologi, Fakultas Sains dan Teknologi, UIN Ar-Raniry Banda Aceh)
Diannita Harahap (Program Studi Biologi, Fakultas Sains dan Teknologi, UIN Ar-Raniry Banda Aceh)



Article Info

Publish Date
28 Apr 2023

Abstract

Thermophilic bacteria are a group of bacteria that grow at a temperature range of 45oC to 80oC and have the potential to produce cellulase enzymes. The tourist area of Ie Suum, Aceh Besar District is one of the hot springs in Aceh. This study aims to observe the morphological characteristics, cellulase enzyme potential and activity test in producing cellulase enzymes from thermophilic bacteria found in Ie Suum. The method of isolating thermophilic bacteria uses the pour plate method and testing the cellulase enzyme activity using the DNS (3,5-dinitrosalicylic Acid) method. 15 isolates were obtained, namely 13 Gram positive and 2 Gram negative, 2 genera were obtained, namely the genus Bacillus sp. and the genus Pseudomonas sp. Based on the potential of the cellulase enzyme, the 15 isolates showed the largest clear zone diameter, namely isolate TS6 3,67 cm, isolate TS7 2,33 cm, and isolate TS10 4,00 cm. The results of measuring enzyme activity using the DNS method obtained isolates TS6 4,7 x 10-3 U/mL, isolates TS7 4,4 x 10-3 U/mL, and isolates TS10 5,5 x 10-3 U/mL.

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Journal Info

Abbrev

kenanga

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Description

KENANGA Journal of Biological Sciences and Applied Biology is a peer-reviewed open access journal that publishes study results (original research articles and review articles). This journal covers all areas of biological science research and applied biology research such as Botany Zoology ...