Biology, Medicine, & Natural Product Chemistry
Vol 12, No 2 (2023)

Bio-larvicidal Potential of Betel Leaves (Piper betle L) Ethanolic Extract in Addition of PEG 400 Diluent on Aedes aegypti Larvae

Listiana Masyita Dewi (Universitas Muhammadiyah Surakarta)
Hilda Zaniba Ariffah (Universitas Muhammadiyah Surakarta)
Riandini Aisyah (Universitas Muhammadiyah Surakarta)
Nurhayani Nurhayani (Universitas Muhammadiyah Surakarta)

Article Info

Publish Date
15 Aug 2023


Dengue hemorrhagic fever (DHF) is a kind of vector transmitted disease, by Aedes aegypti. It is one of major public health problem around the world, including Indonesia, because it may lead to epidemics and death in a short time. The use of plant extracts as bio-larvicidal is thought to be a promising solution, and one of them is the betel leaves (Piper betle L). The addition of polyethylene glycol (PEG) as a diluent is thought may increase the dispersity of plant extract in the water which is larval medium of growth. Objectives: To determine the bio-larvicidal potential of 95% ethanolic extract of betel leaves (EEBL) in addition of PEG 400 diluent on the Aedes aegypti larval mortality. Material and Method: Betel leaves were extracted by maceration using 95% of ethanol. There are two kinds of EEBL concentration used, 0.2% dan 0.4%. PEG 400 was also added as diluent. The samples in this study were Aedes aegypti larvae at instar III-IV, with a total of 400 larvae. Evaluation was performed at 6, 12, 18, and 24 hours. The data obtained was then analyzed by Kruskall Wallis test and post-hoc Mann Whitney test. Result: In all of treatment groups, larval mortality was reached 100% at 24 hours. From the Kruskall Wallis test, p-value obtained was <0.05. From post-hoc Mann Whitney test, the p-value obtained in the comparation between treatment groups and positive control group was >0.05, and the p-value obtained in the comparation between treatment groups and negative control group was <0.05. Conclusion: EEBL in addition of PEG 400 diluent is potential as bio-larvicidal on Aedes aegypti larvae. It is also known that EEBL at concentration of 0.2% and 0.4% in addition of PEG 400 are as effective as temephos as larvicides on Aedes aegypti larvae.

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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health


BIOLOGY, MEDICINE, & NATURAL PRODUCT CHEMISTRY, this journal is published to attract and disseminate innovative and expert findings in the fields of plant, animal, and microorganism secondary metabolite, and also the effect of natural product on biological system as a reference source for ...