Jurnal Kedokteran Hewan
Vol 17, No 2 (2023): June

EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER

Fitrine Ekawasti (National Research and Innovation Agency, Bogor 16114, West Java, Indonesia)
Zul Azmi (Indonesian Goat Research Station, Galang 20585, North Sumatera, Indonesia)
Didik Tulus Subekti (Center for Biomedical Research, National Research and Innovation Agency, Bogor 16114, West Java, Indonesia)
muhammad ibrahim desem (National Research and Innovation Agency, Bogor 16114, West Java, Indonesia)
Arifin Budiman Nugraha (Unknown)
Siti Sa’diah (Unknown)
Umi Cahyaningsih (Unknown)

Article Info

Publish Date
09 Jun 2023

Abstract

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that  stored on liquid nitrogen was isolated using DNAzol™ reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.

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Journal Info
Jurnal Kedokteran Hewan
Website

Abbrev

JKH

Publisher

Universitas Syiah Kuala

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian ...