Jurnal Kedokteran Hewan
Vol 11, No 1 (2017): March

MOLECULAR EXPRESSION OF WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER 4 GENE USING Escherichia coli BL21

Agung Janika Sitasiwi (Biology Department Faculty Science and Mathematic Diponegoro University, Semarang, Indonesia)
Wayan Tunas Artama (Biochemistry Department School of Veterinary Medicine Gadjah Mada University, Yogyakarta, Indonesia)
Agung Budiyanto (Reproduction Department School of Veterinary Medicine Gadjah Mada University, Yogyakarta, Indonesia)
Edy Dharmana (Parasitology Department School of Medicine Diponegoro University, Semarang, Indonesia)

Article Info

Publish Date
07 Apr 2017

Abstract

This research was conducted to find out the Wnt4 recombinant proteins which expressed by Escherichia coli (E. coli) BL21 carrying the recombinant DNA wnt4 (E. coli transformation). Research materials were E. coli BL21 transformation and E. coli BL21 non-transformation (negative control). The expression of recombinant protein was conducted by culturing E. coli for 24 hours in Luria-Bertani (LB) media with isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. Recombinant protein was isolated by sonication of pellet bacteria. Protein analysis performed by 15% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that recombinant protein with a molecular weight of 33 kDa has been expressed by E. coli BL21 transformation successfully. 

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Journal Info
Jurnal Kedokteran Hewan
Website

Abbrev

JKH

Publisher

Universitas Syiah Kuala

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian ...