Jurnal Farmasi Indonesia
Vol 20 No 1 (2023): Jurnal Farmasi Indonesia

Elimination of the Ultraviolet Spectrophotometric-Based Limitation on the Quantification of Metformin HCl in Acid-Stage Medium for a Comparative Dissolution Testing

Syaiful Choiri (Universitas Sebelas Maret)
Dwi Larasati (Diploma Program of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Madani, Yogyakarta, Indonesia 55792)
Ilham Kuncahyo (Faculty of Pharmacy, Universitas Setia Budi)

Article Info

Publish Date
30 Aug 2023

Abstract

Herein, this work aimed to develop an ultraviolet spectrophotometric assay for metformin HCl in comparative dissolution testing along with the acid stage medium due to low molar absorptivity. Metformin HCl was incorporated into the immediate tablet formulation to mimic the analytical matrix system. The dissolution validation procedure was carried out by linearity, accuracy, precision, the limit of quantification, filter compatibility, and aliquot stability that fulfil the USP method for validation of dissolution procedure, particularly assay of the analyte.The results revealed that metformin HCl had difficulty quantifying in acid pH due to the absence UV-band peak. The addition of pH-shifting agents promoted better performance for metformin quantification. Therefore, the method was successfully developed along with R2, predicted R2, accuracy, and precision of 0.9999, 0,9998, 100.57%, and 1.27%, respectively. In addition, it had the minimum placebo interference (0.47%) and was stable for long-term storage under determined conditions.

Copyrights © 2023




Citation Download
RIS
EndNote, Reference Manager, ProCite
BibTex
Latex, Jabref
Original Source
Download Original
Google Scholar
Check in Google Scholar
Journal Info
Jurnal Farmasi Indonesia
Website

Abbrev

farmasi-indonesia

Publisher

Universitas Setia Budi

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Medicine & Pharmacology

Description

Jurnal Farmasi Indonesia (e-ISSN : 2302-4291 dan ISSN : 1693-8615) merupakan jurnal yang dikelola oleh Fakultas Farmasi, Universitas Setia Budi yang terbit sejak Februari 2004 dan terbit 2 kali dalam setahun yaitu periode bulan Maret dan November. Jurnal Farmasi Indonesia menerima naskah tentang ...